TABLE 3.

Expression of the PPA3309-lacZ reporter gene fusion in P. aeruginosa wild type and mutants

P. aeruginosa strainβ-Galactosidase activity (Miller units) or fold inductiona
AerobicFold inductiondAnaerobicFold inductione
Exponential phasebStationary phasecExponential phasebStationary phasec
Wild type214.4 ± 9.61,149.2 ± 70.45.41,339.1 ± 171.72,377.5 ± 270.81.8
anr mutant218.1 ± 8.0914.1 ± 23.54.2NDfNDND
rpoS mutant284.3 ± 164.11,160.5 ± 500.14.1956.2 ± 117.62,262.4 ± 02.4
gacA mutant229.7 ± 11.3985.4 ± 47.04.31,161.2 ± 118.82,524.9 ± 154.42.2
rhlR mutant248.5 ± 4.01,342.2 ± 87.55.41,314.8 ± 56.32747.9 ± 818.22.1
lasR mutant217.1 ± 2.91,139.8 ± 79.45.31,172.6 ± 44.82,106.6 ± 65.51.8
relA mutant455.5 ± 20.81,577.5 ± 70.03.5NDNDND
  • a Shown are the β-galactosidase activities of the PPA3309-lacZ reporter gene fusion in wild-type PAO1 and various mutants under aerobic and anaerobic conditions as described in Materials and Methods.

  • b Exponential growth phase at an OD578 of 0.5 or 0.3 (aerobic and anaerobic culture, respectively).

  • c Stationary phase represents 16 h after entry into the stationary phase.

  • d Fold induction from aerobic exponential phase to aerobic stationary phase.

  • e Fold induction from anaerobic exponential phase to anaerobic stationary phase.

  • f ND, not determined.