TABLE 1.

Bacterial strains used in this study

StrainCharacterizationSource or reference
E. coli
    XL2-BluerecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10(Tetr) Amy Camr]Stratagene
    DH5αdeoR endA1 gyrA96 hsdR17(rK mK+) recA1 relA1 supE44 λthi-1 Δ(lacZYA-argF)U16918
L. monocytogenes
    EGDSv1/2a, wild-typeS. H. E. Kaufmann
    EGD-eDerivative of EGDG. B. Mackaness
    Pkp1Sv1/2a ΔplcA-hly-mpl-actA-plcB, Kanr10
    EGDΔglpD (lmo1293)In-frame deletion of glpD (lmo1293)This study
    EGDΔ1538In-frame deletion of lmo1538This study
    EGDΔ1538ΔglpD (lmo1293)Double mutant of lmo1538 and glpD (lmo1293)This study
    EGD-eΔ1968-1974In-frame deletion of the gene cluster lmo1968-1974 specific for L. monocytogenesThis study