Interfacing transcriptome analysis with attenuated insertion-duplication mutagenesis mutants

Embedded Image
  • a Arrows mark regulated genes probably organized in an operon, and the direction indicates the direction of transcription.

  • b RTL, relative transcript level. Numbers in italics indicate ratios that were determined by SAM not to be significant but showed a consistent up-regulation of ≥1.6.

  • c Mean percentage of intracellular replication (IR) and whose mutation led to attenuation describes the degree of attenuation of the respective mutant with respect to a control group of 107 insertion mutants (cf. Fig. 3). The genes that are intracellularly up-regulated, which are shown in bold type.

  • d Genes specific for L. monocytogenes.

  • e Genes clustered in a 55-kb fragment involved in vitamin B12 synthesis and the coenzyme B12-dependent degradation of 1,2-propanediol and ethanolamine.

  • f Genes that contain a cre box in their promoter region with up to two mismatches to the consensus sequence reported recently (29).

  • g PP cycle, pentose phosphate cycle.