TABLE 1.

Genes showing ≥2-fold-altered mRNA ratios in the C. glutamicum ΔphoRS mutant 10, 30, or 60 min after a shift from Pi excess to Pi limitation

C. glutamicum locus tag no.NCBI no.AnnotationcGenemRNA ratio (after shift/ before shift to Pi-limiting conditions)a
10 min30 min60 min
cg0160NCgl0123Hypothetical protein0.43b0.340.65
cg0229NCgl0181Glutamine 2-oxoglutarate aminotransferase, large subunit gltB 1.281.952.59b
cg0230NCgl0182Glutamine 2-oxoglutarate aminotransferase, small subunit gltD 6.41b5.83b6.24b
cg0310NCgl0251Catalase katA 3.49b3.24b1.55b
cg0464NCgl0375Copper-exporting P-type ATPase ctpA 10.32b21.515.16b
cg0467NCgl0378Put. hemin ABC transporter, periplasmic binding protein hmuT 0.42b0.400.63b
cg0468NCgl0379Put. hemin ABC transporter, permease hmuU 0.330.83b
cg0469NCgl0380Put. hemin ABC transporter, ATPase hmuV 0.41b0.500.69
cg0470NCgl0381Conserved secreted protein0.16b0.22b0.58
cg0500NCgl0405Transcriptional regulator, LysR family2.07b2.742.21b
cg0569NCgl0465Cation-transporting P-type ATPase8.60b4.772.65b
cg0589NCgl0482Put. Fe3+ siderophore ABC transporter, ATPase0.06b0.10b0.44
cg0590NCgl0483Put. Fe3+ siderophore ABC transporter, permease0.22b0.120.51
cg0591NCgl0484Put. Fe3+ siderophore ABC transporter, permease0.15b0.210.26
cg0759NCgl0628Methylcitrate dehydratase prpD2 0.33b0.47b0.55b
cg0760NCgl0629Methylisocitrate lyase prpB2 0.37b0.53b0.49b
cg0762NCgl0630Methylcitrate synthase prpC2 0.22b0.390.40
cg0767NCgl0635Put. siderophore-interacting protein0.16b0.180.62
cg0768NCgl0636Put. Fe3+ siderophore ABC transporter, ATPase0.25b0.090.36
cg0769NCgl0637Put. Fe3+ siderophore ABC transporter, permease0.16b0.090.23
cg0771NCgl0639Put. Fe3+ siderophore ABC transporter, periplasmic binding protein0.14b0.100.29
cg0812NCgl0678Acetyl/propionyl-CoA carboxylase, beta subunit dtsR1 0.26b0.24b0.48b
cg0921NCgl0773Put. siderophore-interacting protein0.13b0.12b0.51b
cg0922NCgl0774Put. Fe3+ siderophore ABC transporter, periplasmic binding protein0.11b0.12b0.27b
cg0924NCgl0776Put. Fe3+ siderophore ABC transporter, periplasmic- binding protein0.07b0.10b0.18b
cg0926NCgl0777Put. Fe3+ siderophore ABC transporter, permease1.240.17
cg0927NCgl0778Put. Fe3+ siderophore ABC transporter, permease0.24b0.28b0.38b
cg0928NCgl0779Put. Fe3+ siderophore ABC transporter, ATPase0.65b0.120.82
cg0957NCgl0802Fatty acid synthase fas-1B 0.460.160.27b
cg1120NCgl0943Transcriptional regulator, AraC-family ripA 0.21b0.200.42b
cg1121NCgl0944Conserved membrane protein0.49b0.921.26
cg1343NCgl1141Nitrate reductase, beta subunit narH 4.40b1.911.08b
cg1344NCgl1142Nitrate reductase, alpha subunit narG 2.26b1.871.13
cg1405NCgl1200Put. siderophore-interacting protein0.34b0.170.46
cg1412NCgl1205Sugar ABC transporter, permease0.48b0.790.91
cg1413NCgl1206Sugar ABC transporter, periplasmic binding protein0.40b0.780.54
cg1418NCgl1209Put. Fe3+ siderophore periplasmic binding protein0.22b0.250.50b
cg1419NCgl1210Transporter, bile acid:Na+ symporter family (TC 2.A.28)0.39b0.350.48
cg1447NCgl1232Put. Co/Zn/Cd exporter, cation diffusion facilitator family (TC 2.A.4)7.166.087.18b
cg1695NCgl1444Put. DNA binding protein3.17b2.182.05b
cg1737NCgl1482Aconitase acn 1.873.69b2.25b
cg1785NCgl1521Ammonia permease amt 1.772.07b4.46
cg1930NCgl1646Put. secreted trypsin-like serine protease0.14b0.21b0.51b
cg2118NCgl1859Transcriptional regulator of sugar metabolism, DeoR family4.08b0.930.69b
cg2120NCgl1861Enzyme II of fructose phosphotransferase system ptsF 3.64b0.660.41b
cg2181NCgl1915Oligopeptide ABC transporter, periplasmic binding protein oppA 0.68b0.53b0.31b
cg2183NCgl1917Oligopeptide ABC transporter, permease oppC 0.710.580.44b
cg2234NCgl1959Put. Fe3+ siderophore binding protein0.26b0.240.55b
cg2283NCgl2001Conserved hypothetical protein0.33b0.360.62
cg2422NCgl2127Lipoate-protein ligase lipB 1.99b2.171.46b
cg2423NCgl2128Lipoic acid synthase lipA 1.65b2.12b1.26b
cg2726NCgl2393Put. membrane protein15.60b3.464.46b
cg2743NCgl2409Fatty acid synthase fas-IA 0.42b0.390.46b
cg2777NCgl2434Put. membrane protein0.47b0.370.55b
cg2782NCgl2439Ferritin ftn 18.82b17.8912.14b
cg2843NCgl2483Phosphate ABC transporter, ATPase pstB 1.02b1.191.94b
cg2844NCgl2484Phosphate ABC transporter, permease pstA 1.06b1.932.43b
cg2845NCgl2485Phosphate ABC transporter, permease pstC 1.39b3.555.21b
cg2846NCgl2486Phosphate ABC transporter, binding protein pstS 1.74b2.554.10b
cg2925NCgl2553Enzyme II of sucrose phosphotransferase system ptsS 3.83b0.93b0.56b
cg3116NCgl2717Phosphoadenosine phosphosulfate reductase cysH 2.53b0.95
cg3118NCgl2718Sulfite reductase (hemoprotein) cysI 3.17b1.410.79
cg3132NCgl2731Put. membrane protein2.53b1.001.13b
cg3195NCgl2787Flavin-containing monooxygenase0.40b0.530.88
cg3227NCgl2817Put. l-lactate dehydrogenase (quinone) lldD 18.31b11.821.32
cg3281NCgl2859Cation-transporting P-type ATPase3.77b2.031.47b
cg3282NCgl2860Put. secreted lipoprotein14.95b4.274.54
cg3286NCgl2864Put. secreted lipoprotein3.54b2.442.67b
cg3287NCgl2865Put. secreted multicopper oxidase4.13b2.811.57
cg3303NCgl2877Transcriptional regulator, PadR-like family7.37b4.164.18b
cg3327NCgl2897Starvation-induced DNA protection protein dps 3.76b3.512.44b
cg3335NCgl2904Malic enzyme mez 0.46b0.450.38b
cg3386NCgl2952Put. maleylacetate reductase tcbF 0.41b0.370.49
cg3387NCgl2953Put. myo-inositol:H+ symporter (TC 2.A.1.1.26)0.32b0.320.50
cg3390NCgl2956Put. sugar phosphate isomerase/epimerase0.38b0.581.15b
cg3391NCgl2957Myo-inositol 2-dehydrogenase idhA1 0.41b0.430.38
cg3404NCgl2970Put. Fe3+ siderophore periplasmic binding protein0.06b0.07b0.18
  • a The mRNA ratios are averages from at least two of three experiments. Only values for open reading frames whose mRNA ratio was altered at least twofold either 10, 30, or 60 min after the Pi downshift are shown.

  • b P < 0.05 as determined by a t test.

  • c Put., putative; CoA, coenzyme A.