TABLE 1.

Bacterial strains and plasmids used in the present study

Strain or plasmidRelevant descriptionReference or source
E. coli strains
    DH5α(λpir) (= CVC23)Supplies R6K π proteinM. Waldor
    SM10(λpir) (= CVC201)thi thr leu tonA lacY supE recA::RP4-2-Tc::Mupir) pro endA hsdA hsdR supF7
    BW25113lacIq rrnB3ΔlacZ4787 hsdR514 ΔaraBAD567 ΔrhaBAD56818
    BW27750 (= BR8701)BW25113 ΔaraFGH φ(ΔaraEp kan PCP18-araE): araE promoter replaced with constitutive promoter CP1818
V. cholerae strains
    CVC209N16961StrrM. Waldor
    CVC239N16961Strr PBADlacI-eyfp λcI-ecfp araE tetR SpcrThis study
    CVC243Same as CVC239 except for 64× lacO in chrIIThis study
    CVC244Same as CVC239 except for 64× λOL1 in chrIThis study
    CVC269Same as CVC243 except for 64× λOL1 in chrIThis study
    CVC270Same as CVC239 except for 64× lacO in chrIThis study
    CVC271Same as CVC239 except for 128× lacO in chrIThis study
    CVC272Same as CVC239 except for 64× λOL1 in chrIIThis study
    CVC300N16961Strr PCP18araE kanThis study
    CVC301N16961Strr PCP18araEThis study
    CVC250N16961recA::kanD. Pal
Plasmids
    pCP20pSC101rep(Ts) Ampr; source of FLP recombinase4
    pDS132R6K γori, mobRP4 sacB Cmr; suicide vector for conjugal transfer and integration31
    pLAU85pBAD24 encoding LacI-ECFP and FtsZ-EYFP; Ampr20
    pPS9Coordinates 1512931-1516681 of chrI cloned in pDS132This study
    pPS10Coordinates 462660-464883 of chrII cloned in pDS132This study
    pPS11pDS132 + vca0527::64× lacO KanrThis study
    pPS17pZC496 + araE SpcrThis study
    pPS18Coordinates 311001-313800 of chrII cloned in pDS132This study
    pPS19pDS132 PBADlacI-eyfp λcI-ecfp PCP18araE tetR SpcrThis study
    pPS20pDS132 PCP18araE KanrThis study
    pPS30pUC19 + 128× lacO KanrThis study
    pPS33pDS132 + vca0527::128× lacO KanrThis study
    pPS41pDS132 + vc1418::64× λOL1 AmprThis study
    pPS42pDS132 + vca0527::64× λOL1 AmprThis study
    pPS50pDS132 + vc1418::64× lacO KanrThis study
    pRFB110pUC19 + 64× lacO Kanr9
    pRFB122pUC19 + 64× λOL1 Cmr9
    pZC496pSC101 PBADlacI-eyfp λcI-ecfp tetR SpcrDon Biek
    pZE12-lucSource of Ampr cassette26