TABLE 1.

Strains, plasmids, and PCR primers used in this study

Strain, plasmid, or primerRelevant characteristic(s)Source or reference
Strains
    14028Wild-type Salmonella enterica serovar TyphimuriumAmerican Type Culture Collection
    WK214028 ΔsdiAThis study
    MM32Vibrio harveyi luxN::cm luxS::Tn5 (Cmr, Kanr)15
Plasmids
    pCS26Low-copy luxCDABE reporter vector (Kanr)9
    pSDIApCS26 containing 14028 sdiA promoterThis study
    pRCKpCS26 containing 14028 rck operon promoterThis study
    pAB13pCS26 containing 14028 pfs promoter7
    pLSRpCS26 containing 14028 lsr operon promoterThis study
    pSIG70-16pCS26 containing σ70-responsive synthetic promoterK. Pabbaraju
    pCR2.1-TOPOMulticopy cloning vector (Ampr, Kanr)Invitrogen
    pTZ18RMulticopy cloning vector (Ampr)53
    pTZΔSDIApTZ18R containing the ΔsdiA constructThis study
    pHSG415Low-copy suicide vector (Cmr, Ampr, Kanr)53
    pHSGΔSDIApHSG415 containing the ΔsdiA constructThis study
PCR primers
    PsdiA1GTAAAGGGAGAGCCGAAAGCThis study
    PsdiA2CCGGAGGATAAGTGGTACGAThis study
    Prck1AGTCTCGAGCGACAATGCGTGCTGGAGATGThis study
    Prck2AGTCGGATCCAGCATCGGCAATATCGCGGGThis study
    Plsr1GCCCTCGAGTATCGCTCATTGTCATAACCThis study
    Plsr2GCCGGATCCGTATTGTGACTGATTTGCThis study
    PsdiA5′AAGTCGAATTCGCGGGAACTGTTAGCCAAAGThis study
    PsdiA5′BAGTCGGATCCGCCGCTGTGTCTGATATTGCThis study
    PsdiA3′AAGTCGGATCCTGCAGAAGAAATTCAATGCGThis study
    PsdiA3′BAGTCAAGCTTAAATTTCACGTTCCGCTGTCThis study