TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotypeSource or reference
E. coli strains
    BW25113lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78Barry Wanner
    MC1000araD139 Δ(ara-leu) Δ(codB-lacI) galK16 galE15 relA1 rpsL spoT1E. coli Genetic Stock Center
    EC100D pir-116mcrA Δ(mrr hsdRMS mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) galU galK rpsL nupG pir-116Epicentre
    NovaBlue SinglesendA1 hsdR17(rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′ [proA+B+ lacIq ZΔM15::Tn10]Novagen
    XL1-BluerecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac F′ [proA+B+ lacIqZΔM15::Tn10]Stratagene
    TP1000araD Δ(argF-lac)U169 rpsL relA flbB ptsF devC rbsR mobAB::kanTracy Palmer
    JOEY19MC1000 (λRS45 ytfE-lacZ)This work
    JOEY24MC1000 (λRS45 ytfE-lacZ) yjeB::EZ-Tn5TM <R6Kγori/KAN-2>This work
    JOEY28MC1000 (λRS45 ytfE-lacZ) yjeB::EZ-Tn5TM <R6Kγori/KAN-2>This work
    JOEY30MC1000 (λRS45 ytfE-lacZ) yjeB::EZ-Tn5TM <R6Kγori/KAN-2>This work
    JOEY59MC1000 (λRS45 ytfE-lacZ) yjeB::kanThis work
    JOEY60MC1000 yjeB::kanThis work
    JOEY61MC1000 (λRS45 ytfE-lacZ) ΔyjeBThis work
    JOEY62MC1000 ΔyjeBThis work
    JOEY72MC1000 (λRS45 hmpA-lacZ)This work
    JOEY73MC1000 (λRS45 ygbA-lacZ)This work
    JOEY75MC1000 (λRS45 tehA-lacZ)This work
    JOEY76MC1000 ΔyjeB (λRS45 hmpA-lacZ)This work
    JOEY77MC1000 ΔyjeB (λRS45 ygbA-lacZ)This work
    JOEY79MC1000 ΔyjeB (λRS45 tehA-lacZ)This work
    JOEY83MC1000 (λRS45 yjeB-lacZ)This work
    JOEY84MC1000 ΔyjeB (λRS45 yjeB-lacZ)This work
Plasmids
    pRS415lacZ fusion vectorValley Stewart
    pSTBlue-1Cloning vectorNovagen
    pKD4Barry Wanner
    pCP20Barry Wanner
    pGIT1205-bp ytfE promoter fragment in pSTBlue-1This work
    pGIT2354-bp hmpA promoter fragment in pSTBlue-1This work
    pGIT3292-bp ygbA promoter fragment in pSTBlue-1This work
    pGIT5232-bp tehA promoter fragment in pSTBlue-1This work
    pGIT8205-bp ytfE promoter fragment with ΔA deletion in pSTBlue-1This work
    pGIT9yjeB and its 5′ noncoding region cloned in pSTBlue-1This work