TABLE 1.

Bacterial strains and plasmidsa

Strain or plasmidRelevant genotype or characteristic(s)Source or reference
Strains
    M90TWild-type S. flexneri serotype 5a32
    HND115M90T ΔphoN2; susceptibleThis work
    HND201M90T ΔospB; susceptibleThis work
    HND215M90T ΔospBphoN2; susceptibleThis work
    HND43M90T ΔmxiA; susceptibleThis work
    HND53M90T ΔvirB::aphA-3; KmrThis work
    HND549M90T ospB-3×FLAG; susceptibleThis work
    HND5311M90T ΔmxiA ospB-3×FLAG; susceptibleThis work
    SC560M90T ΔicsA::Ω; Smr10
    M15[pREP4]E. coli K-12 strain carrying plasmid pREP4Qiagen
Plasmids
    pGEM-TCloning vector, AprPromega
    pBluescript SKCloning vector, AprStratagene Inc., La Jolla, CA
    pBAD28Arabinose-inducible PBAD expression vector, Apr Cmr13
    pSUB11Template plasmid carrying a 3×FLAG epitope and Kmr cassette42
    pKD46λ Red helper plasmid; oriR101 repA101(Ts) P-araB-gam-bet-exo Apr7
    pKD4Template plasmid carrying a Kmr gene with FLP recognition target sequence7
    pCP20FLP helper plasmid; pSC101 replicon (Ts) bla cat Flp (λRp) cI857 Apr Cmr7
    pREP4lacI, KmrQiagen
    pQE30Cloning vector; AprQiagen
    pHN301pQE30 carrying His6-phoN2; AprThis work
    pHN28pBAD28 carrying phoN2; Apr CmrThis work
    pHN111pBAD28 carrying mxiA; Apr CmrThis work
    pFB1-4pBluescript SK carrying a mutated phoN2 gene of EIEC strain HN280 presenting the R192P amino acid substitution36
  • a Apr, ampicillin resistance; Cmr, cloramphenicol resistance; Kmr, kanamycin resistance.