TABLE 2.

Transcript level comparison of S. aureus genes involved in anaerobic metabolic pathways between real-time PCR analysis and microarray analysisa

GenemRNA level changePrimer sequence (sense, antisense)
MicroarrayReal-time PCR
SA0204 (pflB)25.1107.3 ± 50.55′-AAAGCAGGCGTTATTACTGAAAGC-3′
5′-CGTCAATACCTACACCACCGATAG-3′
SA0205 (pflA)33.281.8 ± 38.15′-TGACAAACATATTAGATTGACAGGAAAGC-3′
5′-ATCATCAGAATAACCAGGCACAAGG-3′
SA1094 (cydA)8.418.5 ± 8.15′-TCTCAGCCTTCTTCATTACTTCAG-3′
5′-ACAAATGCCATCGTCATACCG-3′
SA1095 (cydB)9.024.8 ± 8.05′-AGTACCAGGTTCAATAGCACTGATTATG-3′
5′-TGCCAAGAATACTACAGACCAAGC-3′
SA1181 (arcB)26.247.4 ± 16.65′-AGACTTTTCACGACAAGAGGTAG-3′
5′-TGCCATCATACATTCCACCAAG-3′
SA1182 (arcC)15.422.3 ± 9.05′-GAAAATCACCTCAAGAACAACTC-3′
5′-TGTAATTGATAGCCGATGTAAGC-3′
SA2618 (ldh)2.23.4 ± 1.35′-GGTGAGCATGGTGATACTGAAC-3′
5′-TCCATAGTATGTTGACCCTTTAGC-3′
SA2634 (nrdG)5.26.6 ± 1.75′-GTTGACGGTGAAGGAGTAAGATG-3′
5′-AATCCAGTCCATACCCAAATTGTC-3′
SA2635 (nrdD)6.010.4 ± 3.55′-CATCTAATGGACAGACACCTTTTG-3′
5′-ATGTCATAGTTCGGATCTTGCG-3′
16S rRNA5′-GCGAAGAACCTTACCAAATC-3′
5′-CCAACATCTCACGACACG-3′
  • a The results shown are the means of three biological replicates with three technical replicates each (± the standard error) for each gene.