TABLE 3.

Increase in Ag43 protein levels in rfaH mutants compared to the corresponding wild-type strains

Strain backgroundRelative expression of Ag43 variantsa
FluORF52IIIORF47V
MG1655 (wild type)1
MG1655rfaH2.75
536 (wild type)11
536ORF52III6.48
536ORF47V6.50
536rfaH2.2617.02
536rfaH ORF52III11.94
536rfaH ORF47V3.73
  • a The signal intensity of an Ag43 variant was normalized to the signal intensity of the same Ag43 variant obtained for the wild-type strain.