TABLE 1.

Strains used in this study

StrainGenotype or descriptionConstruction, reference, or sourcea
JCM1465Prototroph (type species for Bacillus subtilis)JCMb
168trpC2 (Marburg strain)Laboratory stock
QB7103trpC2 crh::aphA3 ptsH1 amyE::(PΔB levD′-lacZ cat)11
GM273trpC2ΔptsGHI::erm5
61884trpC2 aspB6628
84R5trpC2 aspB66 rpoB518
84R2trpC2 aspB66 rpoB218
84R6trpC2 aspB66 rpoB618
84R32trpC2 aspB66 rpoB3218
84R5-UP1trpC2 aspB66 rpoB5 glcP::Tn10This study
84R5-DN1trpC2 aspB66 rpoB5 ymfI::Tn10This study
84R5-DN2trpC2 aspB66 rpoB5 ccpA::Tn10This study
TI122trpC2 aspB66 amyE::(PntdABC-lacZ cat)18
TI122-UP1trpC2 aspB66 glcP::Tn10 amyE::(PntdABC-lacZ cat)84R5-UP1→TI122
TI122R5trpC2 aspB66 rpoB5 amyE::(PntdABC-lacZ cat)18
TI122R5-UP1trpC2 aspB66 rpoB5 glcP::Tn10 amyE::(PntdABC-lacZ cat)84R5-UP1→TI122R5
TI122R5-DN1trpC2 aspB66 rpoB5 ymfI::Tn10 amyE::(PntdABC-lacZ cat)84R5-DN1→TI122R5
TI122R5-DN2trpC2 aspB66 rpoB5 ccpA::Tn10 amyE::(PntdABC-lacZ cat)84R5-DN2→TI122R5
TI127R5trpC2 aspB66 rpoB5 ntdR::neo amyE::(PntdABC-lacZ cat)18
TI130trpC2 aspB66 ntdA::Tn10 amyE::(PntdABC-lacZ cat)TI130R5→TI122
TI130R5trpC2 aspB66 rpoB5 ntdA::Tn10 amyE::(PntdABC-lacZ cat)18
TI138trpC2 aspB66 ΔntdABC::catpUC18-ΔntdABC::cat →61884
TI167trpC2 aspB66 ntdC-pMutinT3-TntdABCpMutinT3-ntdC→61884
TI167R5trpC2 aspB66 rpoB5 ntdC-pMutinT3-TntdABCTI167→84R5
TI168trpC2 aspB66 TntdABC-pMutinT3-glcPpMutinT3-glcP→61884
TI168R5trpC2 aspB66 rpoB5 TntdABC-pMutinT3-glcPTI168→84R5
TI168Δ4trpC2 aspB66 TntdABC-pMutinT3-ΔglcPpMutinT3-ΔglcP→61884
TI168R5Δ4trpC2 aspB66 rpoB5 TntdABC-pMutinT3-ΔglcPTI168Δ4→84R5
TI171trpC2 aspB66 ΔntdABC::cat-pMutinT3-TntdABCpMutinT3-ntdC→TI138
TI171R5trpC2 aspB66 rpoB5 ΔntdABC::cat-pMutinT3-TntdABC84R5→TI171
TI171R2trpC2 aspB66 rpoB2 ΔntdABC::cat-pMutinT3-TntdABC84R2→TI171
TI171R6trpC2 aspB66 rpoB6 ΔntdABC::cat-pMutinT3-TntdABC84R6→TI171
TI171R32trpC2 aspB66 rpoB32 ΔntdABC::cat-pMutinT3-TntdABC84R32→TI171
TI172trpC2 aspB66 ΔntdABC::cat-TntdABC-pMutinT3-glcPpMutinT3-glcP→TI138
TI172R5trpC2 aspB66 rpoB5 ΔntdABC::cat-TntdABC-pMutinT3-glcP84R5→TI172
TI172R2trpC2 aspB66 rpoB2 ΔntdABC::cat-TntdABC-pMutinT3-glcP84R2→TI172
TI172R6trpC2 aspB66 rpoB6 ΔntdABC::cat-TntdABC-pMutinT3-glcP84R6→TI172
TI172R32trpC2 aspB66 rpoB32 ΔntdABC::cat-TntdABC-pMutinT3-glcP84R32→TI172
TI179trpC2 aspB66 ΔptsGHI::erm amyE::(PntdABC-lacZ cat)GM273→TI122
TI179R5trpC2 aspB66 rpoB5 ΔptsGHI::erm amyE::(PntdABC-lacZ cat)TI179→TI122R5
TI184R5trpC2 aspB66 rpoB5 crh::aphA3 amyE::(PntdABC-lacZ cat)QB7103→TI122R5
TI185R5trpC2 aspB66 rpoB5 crh::aphA3 ptsH1 amyE::(PntdABC-lacZ cat)QB7103→TI179R5
TI191R5trpC2 aspB66 rpoB5 ntdR::neo glcP::Tn10 amyE::(PntdABC-lacZ cat)TI127R5→TI122R5-UP1
TI196R5trpC2 aspB66 rpoB5 ptsH1 amyE::(PntdABC-lacZ cat)TI185R5→TI179
  • a Arrows indicate construction by transformation (with congression in some cases).

  • b JCM, Japan Collection of Microorganisms.