TABLE 2.

Arylsulfatase activities of flgP- and flgQ-astA transcriptional fusions in σ28 and σ54 mutants

StrainArylsulfatase unitsa (mean ± SD)
flgP::astAbflgQ::astAc
Wild type282.7 ± 40.029.3 ± 1.1
ΔfliA308.1 ± 22.929.0 ± 0.7
ΔrpoN163.9 ± 1.510.6 ± 0.9
ΔrpoN/vector155.6 ± 6.311.4 ± 0.8
ΔrpoN/rpoN215.4 ± 4.219.1 ± 1.2
  • a Results are from a typical assay with determinations for each sample performed in triplicate. One unit equals the amount of arylsulfatase required to generate 1 nmol of nitrophenol per hour per OD600 unit.

  • b Strains used were SMS341, SMS350, SMS420, SMS524, and SMS526.

  • c Strains used were SMS281, SMS323, SMS335, SMS532, and SMS534.