TABLE 3.

Expression of ntdABC-lacZ in various B. subtilis mutants

StrainRelevant genotypePntdABC-lacZ (U/OD650)a
−NTD+NTD
TI122R5330 ± 47410 ± 74
TI122R5-UP1glcP::Tn10440 ± 98490 ± 130
TI122R5-DN1ymfI::Tn1023 ± 7.2300 ± 8.5
TI122R5-DN2ccpA::Tn1048 ± 33200 ± 92
TI179R5ΔptsGHI150 ± 79220 ± 18
TI184R5crh::aphA3270 ± 62470 ± 14
TI196R5ptsH1170 ± 18430 ± 33
TI185R5crh::aphA3 ptsH126 ± 12180 ± 82
  • a Cells were grown for 15 h at 37°C in S7N medium containing excess (1%) glucose with or without 500 μg/ml NTD. β-Gal activity is expressed in units/cell optical density at 650 nm. The values are the means ± standard deviations of three or more experiments conducted independently.