TABLE 3.

Virulence and physiologically important genes with altered sequences in strain D39 compared to strain R6a

D39 locus tagGeneFunctionSource or referenceb
SPD_0063strHβ-N-AcetylhexosaminidaseSTM (43)
SPD_0065bgaCβ-Galactosidase52
SPD_0080SSURE fibronectin binding21
SPD_0081rr08Response regulator63, 111
SPD_0168ribERiboflavin synthesis71
SPD_0315-SPD_0323ΔcpsCapsule biosynthesis3, 69
SPD_0336pbp1APenicillin-binding protein transglycosylase/transpeptidase49, 64, 90
SPD_0479nusATranscription termination/antitermination18
SPD_0636spxBPyruvate oxidaseSTM/experimental (12, 65, 87, 91, 92, 106)
SPD_0660ftsXABC transporter/cell division102
SPD_0665pyrDADihydro-orotate dehydrogenase (pyrimidine biosynthesis)5
SPD_0773fruAFructose PTSSTM (43)
SPD_0854flpA(pavA)Fibronectin bindingSTM/experimental (47, 64, 95)
SPD_0967murA1Homolog of MurA (first step in murein biosynthesis)19, 70
SPD_1131carBCarbamoylphosphate synthase, heavy subunit (pyrimidine biosynthesis)73
SPD_1337atpAProton-translocating ATPase, F1 α subunit32
SPD_1346HypotheticalSTM (43)
SPD_1461psaBManganese transportExperimental (24, 27, 72, 75, 85)
SPD_1462psaCManganese transportExperimental (24, 27, 72, 75, 85)
SPD_1512secAPreprotein translocase subunit93
SPD_1671amiAOligopeptide transport2, 57
SPD_1740cinACompetence inducedSTM (43)
SPD_1758rpoCRNA polymerase β′ subunit116
SPD_1797ccpACatabolite controlSTM/experimental (37, 53)
SPD_1961Hypothetical (putative transcription regulator)STM (43)
SPD_1965pcpACholine-binding proteinSTM/experimental (43, 101)
SPD_1974Conserved hypotheticalSTM (43)
SPD_1987Hypothetical (fucolectin-related protein)STM (43)
SPD_2005dltAd-Ala ligaseSTM (43)
SPD_2012glpOα-Glycerophosphate oxidase98
SPD_2022clpCStress-related ATPaseExperimental (22, 23, 51, 96, 97)
SPD_2028cbpDMurein hydrolaseSTM/experimental (, 40, 43, 46, 56)
  • a Specific mutations are listed in Tables S1 and S3 in the supplemental material.

  • b Evidence for a role in virulence exists where indicated. STM, signature-tagged mutagenesis.