TABLE 1.

Arylsulfatase activities of flaA-, flgD-, flgE2-, flaB-, and motA-astA transcriptional fusions in ΔflgP and ΔflgQ mutants

StrainArylsulfatase unitsa (mean ± SD)
flaA::astAbflgD::astAcflgE2::astAdflaB::astAemotA::astAf
Wild type133.2 ± 2.7196.4 ± 7.333.5 ± 1.5175.5 ± 6.8174.6 ± 6.7
ΔfliA64.5 ± 7.0NDgNDND179.9 ± 13.6
ΔrpoNND0.6 ± 0.1<0.1 ± 0.1h<0.2 ± 0.1h185.9 ± 10.6
ΔflgR139.1 ± 4.7<0.4 ± 0.3h<0.1 ± 0.1h0.2 ± 0.1181.5 ± 11.7
ΔflgP340.6 ± 8.5275.2 ± 20.578.5 ± 5.0225.7 ± 4.1189.7 ± 16.9
ΔflgQ341.8 ± 15.3275.5 ± 18.978.0 ± 1.0245.5 ± 9.1190.4 ± 7.7
  • a Results are from a typical assay with determinations for each sample performed in triplicate. One unit equals the amount of arylsulfatase required to generate 1 nmol of nitrophenol per hour per OD600 unit.

  • b Strains used were DRH655, DRH1070, DRH1122, SMS360, and SMS407.

  • c Strains used were SMS354, SMS401, SNJ230, SNJ316, and SNJ322.

  • d Strains used were DRH533, DRN536, DRH830, SMS357, and SMS404.

  • e Strains used were DRH665, DRH667, DRH842, SMS364, SMS410.

  • f Strains used were DRH2329, DRH2331, DRH2333, DRH2335, DRH2337, and DRH2342.

  • g ND, not determined.

  • h At least one of the three tested samples had a value below the limit of detection of the assay, which was 0.06 arylsulfatase unit.