TABLE 1.

Strains and plasmids used in this study

Strain or plasmidaRelevant genotype or featuresSource or reference
Strains
    MG1655Wild-typeCGSCb
    HME63W3110 (λcI857Δcro-bioA) mutS::AmpD. L. Court
    JC7623recB21 recC22 sbcB15 sbcC201CGSC
    JH39sfiA11 thr-1 leu-6 hisG4 argE3 ilv(Ts) galK2 srl (?) rpsL31ΔlacU169 dinD1::MudI (Ampr lac)K. Kreuzer (35)
    MR2MG1655 ΔlacIThis study
    MR10MR2 ΔftsEX210::KanThis study
    MR11MR10/pMN6This study
    MR12MR2 ftsE211::Tn10dCmThis study
    MR14MR12/pMN6This study
    MR19MR12/pMN2This study
    MR20MR19 tatB::Tn10dKanThis study
    MR21MR2 ΔsufI21::KanThis study
Plasmids
    pACYC184p15A-based, Cmr, TetrLab collection
    pAM34pMB1-based, IPTG-dependent replicon, Ampr, Spcr15
    pCL1920pSC101-based; Spcr27
    pKRP11ColEI-based; source of a portable Kanr cassette flanked by multiple cloning sites41
    pMA2pSC101(Ts), AmprJapanese cloning vector collection
    pUC4KColE1-based; source of the Kanr cassetteLab collection
    pALS10pBR322-relA45
    pALS13pBR322-relA′45
    pJK537pBR322-dksA21
    pMN2pMA2-ftsYEXThis study
    pMN4pCL1920-ftsYEXThis study
    pMN6pAM34-ftsYEXThis study
    pMN8pCL1920-ftsQAZThis study
    pMN9pCL1920-ftsQAThis study
    pMN10pCL1920-ftsQThis study
    pMN11pCL1920-ftsAThis study
    pMN12pCL1920-ftsAZThis study
    pMN14pCL1920-ftsNThis study
    pMN15pCL1920-sdiAThis study
    pMN16pCL1920-sufIThis study
    pMN17pACYC184-relA′This study
    pMN18pCL1920 with a 12-kb PstI fragment from λ505 Kohara phage encompassing the sufI geneThis study
  • a All strains are derivatives of E. coli K-12 and are F. The mutant allele numbers for ftsEX or sufI are given according to the registry at the CGSC. Strain MR2 was constructed by transducing a deletion of lacI linked to zai-911::Tn10dCm from strain GJ2422-S42 (6) into MG1655 lacZ4525::Tn10dKan (6) and selecting for Lac+ transductants, followed by screening for LacI, Cms, and Kans colonies.

  • b CGSC, Coli Genetic Stock Center.