TABLE 1.

Bacterial strains and plasmids used for this study

Strain or plasmidRelevant characteristicsaReference
E. coli strains
    DH5αφ80d lacZΔM15 endA1 recA1 hsdR17 (rK mK+) supE44 thi-1 gyrA96 relA1 F Δ(lacZYA-argF)U1694
    S17-1thi pro hsdR recA Tra+56
    KAM3ΔacrB Δ(lac-pro) supE thi hsdΔ5/F′ traD36 proA+ B+ lacIq lacZ ΔM1536
P. aeruginosa strains
    K767PAO1, wild type29
    K1525K767 ΔmexXY58
    K2439K1525 ΔssrAThis study
    K2440K1525 ΔsmpBThis study
    K2413K767 ΔPA5471This study
    K2414K767 ΔmexXY ΔPA5471This study
    K2415K767 ΔmexZThis study
    K2416K767 ΔmexZ ΔPA5471This study
    K2417K767 ΔPA5471-PA5470This study
    K2437K767 ΔssrAThis study
    K2438K767 ΔsmpBThis study
    K2162Clinical isolate displaying MexXY-dependent pan-aminoglycoside resistance58
    K2418K2162 ΔPA5471This study
    K2435K2162 PA5471::mini-Tn5-tetThis study
    K2436K2162 PA5471::mini-Tn5-tetThis study
    YM34PAO1 ΔmexAB ΔmexCD-oprJ ΔmexEF-oprN37
    YM44YM34 ΔmexXY37
Plasmids
    pEX18TcBroad-host-range gene replacement vector; sacB Tcr22
    pYM008pEX18Tc::ΔPA5471This study
    pYM015pEX18Tc::ΔPA5471-PA5470This study
    pYM021pEX18Tc::ΔmexZThis study
    pYM022pEX18Tc::ΔssrAThis study
    pYM023pEX18Tc::ΔsmpBThis study
    pUCP20TBroad-host-range cloning vector; Mob+ Apr Cbr53
    pYM010pUCP20T::PA5471This study
    pYM013pUCP20T::PA5471-PA5470This study
    pYM017pUCP20T::mexZThis study
    pBC KS(+)Phagemid cloning vector; CmrStratagene
    pTEM4pBR322::mexXY; Apr35
    pMMB190Broad-host-range, low-copy-number cloning vector; lacIq Apr Cbr34
    pYM004pMMB190::mexXYThis study
  • a Tcr, tetracycline resistance; Apr, ampicillin resistance; Cbr, carbenicillin resistance; Cmr, chloramphenicol resistance.