TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype and phenotypeSource or reference
Strains
    E. coli
        DH5αλφ 80dlacZΔM15Δ (lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA1Promega
        S17-1λpirrecA thi pro rK mK+ RP4::2-Tc::MuKm Tn7 Tpr Smrλ pir11
    V. cholerae strains
        Fy_Vc_1V. cholerae O1 El Tor A1552; smooth variant; Rifr60
        Fy_Vc_2V. cholerae O1 El Tor A1552; rugose variant; Rifr60
        Fy_Vc_237Fy_Vc_1 mTn7-GFP; Rifr Gmr3
        Fy_Vc_240Fy_Vc_2 mTn7-GFP; Rifr Gmr35
        Fy_Vc_5Fy_Vc_2Δ vpsT ΔlacZ; Rifr6
        Fy_Vc_1956Fy_Vc_5 mTn7-GFP; Rifr GmrThis study
        Fy_Vc_6Fy_Vc_2Δ vpsR ΔlacZ; Rifr6
        Fy_Vc_1955Fy_Vc_6 mTn7-GFP; Rifr GmrThis study
        Fy_Vc_2037Fy_Vc_2 vpsR::pGP704; Rifr Apr57
        Fy_Vc_7Fy_Vc_2Δ vpsR ΔvpsTΔ lacZ; Rifr6
        Fy_Vc_1957Fy_Vc_7 mTn7-GFP; Rifr GmrThis study
        Fy_Vc_2038Fy_Vc_2Δ vpsT ΔlacZ vpsR::pGP704; Rifr AprThis study
        Fy_Vc_1951Fy_Vc_2 hapR::pGP704; Rifr Apr59
        Fy_Vc_1958Fy_Vc_1951 mTn7-GFP; Rifr Gmr AprThis study
        Fy_Vc_1952Fy_Vc_2Δ vpsT ΔlacZ hapR::pGP704; Rifr AprThis study
        Fy_Vc_1959Fy_Vc_1952 mTn7-GFP; Rifr Gmr AprThis study
        Fy_Vc_1953Fy_Vc_2Δ vpsR ΔlacZ hapR::pGP704; Rifr AprThis study
        Fy_Vc_1960Fy_Vc_1953 mTn7-GFP; Rifr Gmr AprThis study
        Fy_Vc_1954Fy_Vc_2Δ vpsR ΔvpsTΔ lacZ hapR::pGP704; Rifr AprThis study
        Fy_Vc_1961Fy_Vc_1954 mTn7-GFP; Rifr Gmr AprThis study
        Fy_Vc_343Fy_Vc_2Δ cdgA; Rifr35
        Fy_Vc_359Fy_Vc_343 mTn7-GFP; Rifr Gmr35
        Fy_Vc_1717Fy_Vc_2Δ cdgA ΔvpsT; RifrThis study
        Fy_Vc_1733Fy_Vc_1733 mTn7-GFP; Rifr GmrThis study
        Fy_Vc_1721Fy_Vc_2Δ cdgA ΔvpsR; RifrThis study
        Fy_Vc_1458Fy_Vc_1458 mTn7-GFP; Rifr GmrThis study
        Fy_Vc_1731Fy_Vc_2Δ cdgA ΔhapR; RifrThis study
        Fy_Vc_1725Fy_Vc_2Δ cdgA ΔvpsTΔ hapR; RifrThis study
        Fy_Vc_1446Fy_Vc_2Δ cdgA ΔvpsRΔ hapR; RifrThis study
        Fy_Vc_183Fy_Vc_1Δ hapR; RifrThis study
        Fy_Vc_344Fy_Vc_1Δ cdgA; Rifr35
        Fy_Vc_1675Fy_Vc_1Δ cdgA ΔhapR; RifrThis study
Plasmids
    pGP704-sac28pGP704 derivative; sacB; AprG. Schoolnik
    pGP704Suicide plasmid; Apr41
    pGP704::vpsR57
    pGP704::hapR58
    pCC2pGP704-sac28::ΔlacZ; Apr6
    pCC9pGP704-sac28::ΔvpsT; Apr6
    pCC27pGP704-sac28::ΔvpsR; Apr6
    pFY-9pGP704-sac28::ΔhapR; AprG. Schoolnik
    pFY-149pGP704-sac28::ΔcdgA; Apr35
    pMCM11pGP704::mTn7-GFP; Gmr AprM. Miller and G. Schoolnik
    pUX-BF13oriR6K helper plasmid, provides Tn7 transposition function in trans; Apr2