TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
    GAS
        AL168str-rM22, OF+, streptomycin-resistant derivative of AL16828
        nr4M22, Tn916 mutant of AL168str-r in mga28
        JRS4M6, streptomycin-resistant derivative of D47126
        JRS4-PolHis (12Z05A)M6, derivative of JRS4 with chromosomal His-tagged β′ subunit of RNA polymerase21
        JRS519M6, derivative of JRS4 (mga-10)18
        SF370M1, sequenced strain27
        AP4M4, OF+14
    E. coli
        DH5αhsdR17 recA1 gyrA endA1 relA19
        BL21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Novagen
Plasmids
    pBluescript II KS(−)ColE1 ori Ampr lacZαStratagene
    pCD2PT7A (Bacillus subtilis)5
    pKSM164Pmga-mga-his1
    pKSM170PT7-mga-his1
    pKSM415PrpsL, 3′ HindIII siteThis study
    pKSM416Pemm, 3′ EcoRI siteThis study
    pKSM417PsclA (sequence downstream of Mga-binding site), 3′ XhoI site1
    pKSM418PsclA (ΔMga-binding site), 3′ XhoI site1
    pKSM419PsclA (full length), 3′ XhoI site1
    pKSM420Psof, 3′ BamHI siteThis study