TABLE 1.

Characterization of NCO uptake and metabolism in Synechococcus sp. strain UTEX 625

Experimental conditionsaN14CO uptake (% of control)14C fixation (% of control)
Light (control)100100
Dark6.32.4
Light + 25 μM DCMU7.92.2
Light + 15 mM glycolaldhyde59.15.6
Light + 15 mM oxalate8899.7
Light + 1 mM ethoxyzolamide3932.3
  • a NCO uptake and C fixation were measured by the silicone fluid filtering centrifugation technique using method A. Cells grown in standing culture were incubated with 1 mM KO14CN for 2 min in BTP/HCl buffer, pH 8.0, containing 25 mM NaCl at 30°C and illuminated at 300 μmol m−2 s−1. Data are the average of 5 experiments in triplicate ±6.5%.