TABLE 3.

Complex nutritional requirements of the L. rhamnosus GG luxS mutant CMPG5412 in Lactobacilli AOAC medium

Component addedConcnaμmaxbOD600 at 72 h
Wild type (% of no addition)cCMPG5413 (% of wild type)dWild type (% of no addition)cCMPG5413e (% of wild type)d
None100f30.3 ± 5.9100f33.8 ± 7.8
DPD0.5 mM96.8 ± 1.036.6 ± 9.895.6 ± 2.547.6 ± 9.7
Methionine100 μM98.3 ± 1.228.5 ± 3.996.3 ± 0.127.3 ± 6.8
SAM1 mM86.6 ± 4.637.7 ± 12.788.3 ± 5.242.9 ± 6.3
Cysteine-HCl2 mM102.3 ± 2.672.9 ± 9.0102.6 ± 0.183.5 ± 9.2
Vitamins and cysteineg101.5 ± 2.983.2 ± 3.5102.8 ± 2.890.5 ± 6.9
Folic acid20 nM106.6 ± 1.955.9 ± 13.099.4 ± 0.160.4 ± 1.0
Cyanocobalamin7 nM103.6 ± 1.633.1 ± 7.2101.9 ± 0.935.5 ± 4.7
d-(+)-biotin40 μM99.6 ± 0.661.1 ± 4.896.3 ± 0.861.8 ± 16.8
Ca-d-(+)-pantothenate20 μM111.3 ± 3.074.2 ± 5.8106.6 ± 0.277.6 ± 10.9
Pyridoxol-HCl5 μM100.6 ± 0.637.4 ± 3.298.9 ± 0.143.9 ± 9.6
  • a Concentration that resulted in the best complementation of growth in Lactobacilli AOAC medium.

  • b Maximal growth rate, μmax, where μ equals ln2/g, g equals [(t2t1) log2]/(log OD2 − log OD1), and g equals the doubling time (hours).

  • c Growth of wild-type L. rhamnosus GG in Lactobacilli AOAC medium.

  • d Growth of the luxS mutant was compared to growth of wild-type L. rhamnosus GG under the same conditions.

  • e A large lag phase was still observed when partial complementation could be achieved.

  • f One hundred percent refers to the growth characteristics of wild-type L. rhamnosus GG in Lactobacilli AOAC medium without extra components added.

  • g The vitamin solution contained folic acid (0.01 μg/ml), biotin (10 μg/ml), nicotinic acid (0.1 μg/ml), pantothenic acid (10 μg/ml), riboflavin (0.1 μg/ml), pyridoxol (1 μg/ml), and cyanocobalamin (0.01 μg/ml).