TABLE 5.

Relevant nucleotides in doubly resistant C. trachomatis strains isolated from the indicated flasks

Constructnawt sequence and relevant ntb change
23S rRNA-1 and -2 (nt 2034-2044)cgyrA (nt 243-253)rpoB (nt 1378-1388)
wt ACGAAAAGACC AGAAAGTGTCA ATGGATCAGAC
Cross no. 1
    OFXr-1 ........... ......G.... NS
    LINr-1 .....C..... ........... NS
    OFXr LINr flasks
        Set B2/10 .....C..... ......G.... NS
        Set C10/10 .....C..... ......G.... NS
        Set D10/10 .....C..... ......G.... NS
Cross no. 2
    OFXr-2 ........... ....C...... NS
    LINr-1 .....C..... ........... NS
    OFXr LINr flasks
        Set B1/10d .....C..... ........... NS
1/10 .....C..... ......A.... NS
        Set C10/10 .....C..... ....C...... NS
Cross no. 3
    OFXr-1 NSf ......G.... ...........
    RIFr-1 NS ........... .....G.....
    OFXr RIFr flasks
        Set A5/10e NS ......G.... ...........
        Set B1/10d NS ........... .....G.....
2/10 NS .....T..... .....G.....
        Set C10/10 NS ......G.... .....G.....
Cross no. 5
    OFXr-1 ........... ....C...... NS
    LINr-1 .....C..... ........... NS
    OFXr LINr flasks
        Set V10/10 .....C..... ....C...... NS
  • a Number of flasks yielding isolates with the mutation/total number of flasks.

  • b nt, nucleotide.

  • c The two 23S rRNA genes were individually sequenced (Tables 1 to 3); both of the genes in an isolate always had identical sequences.

  • d Spontaneous OFXr mutant that lacked a mutation in gyrA nt 1 to 438.

  • e Spontaneous RIFr mutants in cross no. 3 that had a G1399→A, T1432→G, T1432→C, G1441→T, or C1525→T mutation in rpoB.

  • f NS, not sequenced.