TABLE 2.

Effect of [12Ci] on N14 CO transport by Synechococcus sp. strain UTEX 625 grown in standing culturea

Experimental condition (with 1 mM KO14CN)% of control value at 20 s% of control value at 2 min
N14CO uptakePool14C fixationN14CO uptakePool14C fixation
0 mM KHCO3100100b100c100100d100e
2 mM KHCO348.2 ± 9.345.4 ± 16.247.5 ± 21.638.4 ± 15.241.0 ± 12.924.9 ± 10.6
5 mM KHCO349.4 ± 7.346.4 ± 19.342.9 ± 12.239.6 ± 14.641.5 ± 18.730.4 ± 19.7
  • a Values were determined using method C in BTP-HCl buffer, pH 8, containing 25 mM NaCl. Light was supplied at 300 μmol of photons m−2 s−1. Data are the average of seven experiments ± standard deviations.

  • b Control value, 6.0 ± 2.0 mM.

  • c Control value, 0.06 μmol of C mg−1 Chl.

  • d Control value, 11.0 ± 7.0 mM.

  • e Control value, 0.23 μmol of C mg−1 Chl.