TABLE 1.

Strains, plasmids and primers used in this study

Strain, plasmid, or primerGenotype or descriptionReference or source
Strains
    E. coli EL250F mcr Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG [λcl857 araC-PBADflpe]30
    S. aureus
        RN4220Restriction-deficient transformation recipientLaboratory stock
        SH1000Wild-type strain cured of prophages; rsbU+20
        KC055katA::pAZ106 katA+This study
        KC056perR::kan katA::pAZ106 katA+This study
        KC067ahpC::tet katA::pAZ106 katA+This study
        KC070ahpC::tet perR::kan katA::pAZ106 katA+This study
        KC041ahpC::tetThis study
        KS100katA::Tn917 (ery)53
        KC043ahpC::tet katA::Tn917 (ery)This study
        MHKAsodA::Tn917 (ery)26
        KC075ahpC::tet sodA::Tn917 (ery)This study
        KC072ahpC::tet katA::Tn917 (ery) perR::kanThis study
        MHK2fur::tetLaboratory stock
        KC044ahpC::tet fur::kanThis study
        KC073katA::Tn917 (ery) fur::kanThis study
        KC072ahpC::tet fur::kan katA::Tn917 (ery)This study
        GC3ahpC::tet katA::Tn917 (ery) pSK5630This study
        GC2ahpC::tet katA::Tn917 (ery) pSK5630 (ahpC+)This study
Plasmids
    SABAC1S. aureus bacterial artificial chromosome containing genomic DNA from 457727 to 401407 (COL coordinates)J. Iandolo, http://www.genome.ou.edu/staph.html
    pGL400tet cassette-containing vectorJ. Garcia-Lara, personal communication
    pSK5630E. coli-S. aureus shuttle vector12
    pKC2AhpC + 1-AhpC + 2 ahpC gene and promoter region in pSK5630This study
Primers
    AhpCtet1AACAAAGAAATCTTACCATTTACAGCGCAAGCTTTCGATCCACGGATTTTATGACCGATGAGAAGThis study
    AhpCtet2AATCTAAACCAGGTTGCAATGTTTTAGCGCCTTCTTCCCAAGAAATCCCTTTGAGAATGTTTThis study
    AhpC + 1AATGTTGGATCCTGTCAGTGAAATGAATGThis study
    AhpC + 2TCACTCGGATCCTTCATCCATCTCGAACThis study
    Acn1TAAGCGTTTTCTAAGCATGATGThis study
    Acn3ACGTGATGACATATAGTTTTTAGThis study