TABLE 2.

Transduction frequencies of β-lactam-induced SaPI-containing lysogenic strainsa

Donor strainPhageSaPIInducerbTransduction titerc
JP44φ80αSaPIbov1MC7.2 × 109
A2.7 × 107
P3.0 × 107
CL1.6 × 107
CE3.2 × 107
NI8.1 × 104
JP47φ11SaPIbov1MC2.1 × 106
A6.0 × 104
P5.8 × 104
CL4.4 × 104
CE5.6 × 104
NI1.7 × 103
JP50 (recA mutant)φ11SaPIbov1MC<10
A<10
NI<10
JP85 [JP83 LexA (G94E)]φ80αSaPIbov1MC5 × 105
A4 × 104
NI2.3 × 103
JP52φ11 cIG131ESaPIbov1MC<10
A<10
NI<10
RN8667φ80αSaPI1A2.1 × 105
P1.6 × 105
CL2.2 × 105
CE2.9 × 105
NI6.0 × 103
  • a Results from a representative experiment are shown.

  • b MC, mitomycin C (2 μg/ml); A, ampicillin (10 μg/ml); P, penicillin G (10 μg/ml); CL, cloxacillin (10 μg/ml); CE, ceftriaxone (10 μg/ml); NI, not induced.

  • c Number of transductants per milliliter of induced culture, using RN4220 as the recipient strain.