TABLE 1.

Strains and plasmids

Strain or plasmidDescription or constructionSource or reference(s)
Strains
    E. coli
        DH5αF′φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(r m+) deoR thi-1 supE44 λ gyrA96 relA126
        S17-1E. coli 294::RP4-2(Tc::Mu)(Km::Tn7)42
        BL21(λDE3)F ompT hsdSB(rB mB) gal dcm (λDE3)Novagen
    C. crescentus
        NA1000syn-1000; previously called CB15N12
        CB15CB15; wild type31
        CMS1-CMS40Genomic marker strains; Kanr48
        UJ590CB15 ATCC 19089 ΔpilA1
        SU214CB15N rpoN::Tn56
        YB1382CB15N pleC::mini-Tn5 lacZ2 I2913
        YB3059CB15 ΔdivJ::specA. Hinz, unpublished
        YB3202NA1000 ΔdivJ::specThis study
        YB1388NA1000 divJ::mini-Tn5 lacZ2This study
        YB3215NA1000 ΔdivJ::spec sdj-21 (original suppressor)This study
        YB3216NA1000 ΔdivJ::spec sdj-22 (original suppressor)This study
        YB3219NA1000 ΔdivJ::spec sdj-25 (original suppressor)This study
        YB3227NA1000 ΔdivJ::spec sdj-33 (original suppressor)This study
        YB3229NA1000 ΔdivJ::spec sdj-35 (original suppressor)This study
        YB3314NA1000 divL22 (exchanged suppressor)This study
        YB3315NA1000 ΔdivJ::spec divL22 (exchanged suppressor)This study
        YB3316NA1000 divL33 (exchanged suppressor)This study
        YB3317NA1000 ΔdivJ::spec divL33 (exchanged suppressor)This study
        YB3318NA1000 divL35 (exchanged suppressor)This study
        YB3319NA1000 ΔdivJ::spec divL35 (exchanged suppressor)This study
        YB3325NA1000 cckA21 (exchanged suppressor)This study
        YB3326NA1000 ΔdivJ::spec cckA21 (exchanged suppressor)This study
        YB3327NA1000 cckA25 (exchanged suppressor)This study
        YB3328NA1000 ΔdivJ::spec cckA25 (exchanged suppressor)This study
Plasmids
    pNPTS138Used for two-part selection for in-frame deletions4, 46
    pHP45ΩUsed to obtain Ω cassette for spectinomycin resistance32
    pBGST18Used to construct markers4
    pGSZUsed to construct markers, a Gentr derivative of pGMTZ15
    pJS70Used to measure PpilA activity43
    plac290/HB2.0BPUsed to measure PftsZ activity22
    pMSP8LCUsed to measure PftsQA activity38
    pMR20Medium-copy Tetr plasmid36
    pJW375Used to overexpress DivL29952
    pNPTS138UP, DN, ΩUsed to delete divJThis study
    pMR20divJUsed to complement divJ null mutantsThis study
    pBGST18CMS12.3Used to generate genomic marker 3/10 of the way between CMS12 and CMS13This study
    pBGST18CMS12.4Used to generate genomic marker 4/10 of the way between CMS12 and CMS13This study
    pBGST18CMS12.49Used to generate genomic marker 49/100 of the way between CMS12 and CMS13This study
    pBGST18CMS12.54Used to generate genomic marker 54/100 of the way between CMS12 and CMS13This study
    pBGST18CMS12.59Used to generate genomic marker 59/100 of the way between CMS12 and CMS13This study
    pBGST18CMS12.6Used to generate genomic marker 6/10 of the way between CMS12 and CMS13This study
    pBGST18CMS37.65Used to generate genomic marker 65/100 of the way between CMS37 and CMS38This study
    pBGST18CMS37.75Used to generate genomic marker 75/100 of the way between CMS37 and CMS38J. Wagner, unpublished
    pGSZCMS12.3Used to generate genomic marker 3/10 of the way between CMS12 and CMS13This study
    pGSZCMS37.65Used to generate genomic marker 65/100 of the way between CMS37 and CMS38This study