TABLE 2.

Electrophysiological properties of native and recombinant porin proteins in planar lipid bilayers

Porin IBReversal potentialIon selectivityb (PCl/PNa+)
Native wild-type−6.3 ± 0.32.2 ± 0.1
Recombinant wild-type−4.9 ± 1.21.8 ± 0.3
G120K−9.7 ± 0.9a3.7 ± 0.6
G120D A121D−6.4 ± 1.02.2 ± 0.3
G120P A121P−6.5 ± 0.62.3 ± 0.2
G120R A121H−7.5 ± 0.62.6 ± 0.3
  • a Analysis of variance; P < 0.001.

  • b Ion selectivity was derived from the mean reversal potential with the Goldman-Hodgkin-Katz equation (16) (see Materials and Methods).