TABLE 3.

ODC and ADC activity in cellular extracts of Y. pestis speA+ speC+ (KIM6+) and polyamine-deficient mutants

Enzymenmol CO2/min/mg ± SDaμmol CO2/min/mg ± SDa
speA+speC+ΔspeAΔspeCΔspeA ΔspeCΔspeA ΔspeC (pBAD/HisbADC)Purified ADCPurified ODC
ADC20.86 ± 4.222.01 ± 0.8515.36 ± 0.620.94 ± 0.3526.14 ± 0.7729.10 ± 7.93NDb
ODC8.98 ± 1.0219.11 ± 4.920.67 ± 0.130.48 ± 0.39NDbNDb1.89 ± 0.48
  • a Values are the averages of three or more experiments.

  • b ND, not determined.