TABLE 1.

Densitometry scans of immunoreactive cell extracts prepared from ALAB49 at stationary phasea

Cell extract sourceAntiserumValue (pixels) for sum of peaks (% of total) Approx ratio of polymeric to monomeric form
Total laneMigration of ≥75 kDaMigration of <75 kDa
wtFctAb 43,45338,261 (88)5,192 (12)7.4
cpa mutantFctA42,9967,214 (17)35,782 (83)0.2
wtCpa29,92428,152 (94)1,772 (6)15.9
fctA mutantCpa7,209655 (9)6,554 (91)0.1
  • a Based on immunoblot findings in Fig. 4.

  • b Based on overdeveloped panel for anti-FctA serum in Fig. 4.