TABLE 2.

Plasmids used in this study

PlasmidDescriptionReference or source
pABB-CRS2Positive-selection suicide vector31
pDONR201Gateway cloning vectorInvitrogen
pDONR-escCescC cloned into pDONR201This study
pDONR-escDescD cloned into pDONR201This study
pDONR-escJescJ cloned into pDONR201This study
pDONR-GST-escDescD cloned into pDONR201This study
pDONR-GST-escFescF cloned into pDONR201This study
pDONR-escD-V5escD cloned into pDONR201This study
pDONR-escF-V5escF cloned into pDONR201This study
pABB-ΔescCescC deletion in pABB-CRS2This study
pABB-ΔescDescD deletion in pABB-CRS2This study
pABB-ΔescJescJ deletion in pABB-CRS2This study
pTrc99AProkaryotic expression vectorPharmacia
pABB-Trc99cmpTrc99A-derived vector31
p99-escCescC cloned into pABB-Trc99cmThis study
p99-escDescD cloned into pABB-Trc99cmThis study
p99-escJescJ cloned into pABB-Trc99cmThis study
p99-espAespA cloned into pABB-Trc99cm31
pGEX-6P-1GST fusion protein expression vectorAmersham
pGEX-escCescC cloned into pGEX-6P-1This study
pGEX-escJescJ cloned into pGEX-6P-1This study
pDEST15GST fusion protein expression vectorInvitrogen
p15-escDescD cloned into pDEST15This study
p15-escFescF cloned into pDEST15This study
pBAD-DEST49V5-tagged protein expression vectorInvitrogen
p99ccdB-V5V5-tagged protein expression vector derived from pTrc99AThis study
p99-escD-V5escD cloned into p99ccdB-V5This study
p99-escF-V5escF cloned into p99ccdB-V5This study