TABLE 1.

Plasmids and primers used in this study

PlasmidPrimersaSource of template DNAbRelevant features
pBAD22thrB5′-TTAGGAATTCGACATGGTTAAAGTTTATGCCC, 5′-CCTGAAGCTTGTGATCTTTCAGATTGTAGAGTMG1655thrB gene in EcoRI-HindIII sites of pBAD22
pBAD22dnaJ5′-CAATGAATTCAAGATGGCTAAGCAAGATTATTA, 5′-GCAGTCTAGAGGGGAGGTTAGCGGGTCAGMG1655dnaJ gene in EcoRI-XbaI sites of pBAD22
pBAD18pmrC5′-TGGAGAATTCAACATGTTAAAGCGCTT, 5′-TCAGAAGCTTCATTCGCTTAGTCTCCTppmrC (17)pmrC gene in EcoRI-HindIII sites of pBAD22
pBAD22mazF5′-AGGAGAATTCGTAATGGTAAGCCGATACGTA, 5′-ATAGAAGCTTGTTAGTAACACTACCCAATCAGMG1655mazF gene in EcoRI-HindIII sites of pBAD22
pBADCMhipA5′-GTGGGCATGCCTAAACTTGTCACTTGG, 5′-ACCCGAATTCTCACTTACTACCGTATTCTCGMG1655hipA gene in NcoI-EcoRI sites of pBADMycHis; a stop codon (boldface) was introduced to avoid expression of Myc epitope and His tail
  • a Primers used for PCR amplification of the genes cloned in the corresponding plasmids. Restriction sites are underlined.

  • b Template DNA used for PCR amplification of the genes.