TABLE 1.

Bacterial strains used in this study

SpeciesStrainGenotype
S. enterica serovar Typhimurium LT2BE47thr-480::Tn10dCam
BE188ΔpduL670
BE281pta-406::Tn10
BE284ΔpduL670/pLAC22-pduL (Ampr)
BE285ΔpduL670/pLAC22-no insert (Ampr)
BE286pLAC22-pduL (Ampr)
BE287pLAC22-no insert (Ampr)
BE291ΔpduL670 pta-209::Tn10
BE527pta-209::Tn10
BE529pta-209::Tn10/pLAC22-no insert (Ampr)
BE530pta-209::Tn10/pLAC22-pduL (Ampr)
BE548pta-209::Tn10/pBE522-no insert (Kanr)
BE549pta-209::Tn10/pBE522-pta (Kanr)
E. coliBL21(DE3)RIL(E. coli B) FompT hsdS (rB mB) dcm+ Tetr gal l (DE3) endA Hte (argU ileY leuW Camr)
BE554BL21(DE3)RIL/pTA925-pduL-His8 (T7 expression vector containing a minimal clone for production of PduL-his8, Kanr)
BE119BL21(DE3)RIL/pTA925-no insert (Kanr)
S17.1λpirrecA(RP4-2-Tc::Mu) λpir