TABLE 2.

Plasmids used in this study

PlasmidRelevant characteristic(s)aSource or reference
pAX24xcpP to xcpZ cluster in pLAFR319
pBBR1MCS5Gmr; cloning vector; Plac30
pBC18RApr; cloning vector8
pPHO7Apr; phoA without ss-encoding part24
pET16bApr lacINovagen
pCR-LacIpBC18R lacIThis study
pCR-PhoApBC18R; phoA without ss-encoding partThis study
pYRCpBBR1MCS-5 lacIThis study
pYRC-ApYRC; phoA without ss-encoding partThis study
pCRII-TOPOApr Kmr; TOPO TA cloning vectorInvitrogen
pCR-XcpT1-2pCRII-TOPO xcpTThis study
pJGA01pYRC Plac-xcpT-phoAThis study
pMPM-K4ΩKmr; cloning vector; ParaBAD34
pCR-XcpT1-3pCRII-TOPO xcpT (start codon replaced by NcoI site)This study
pJGA07pMPM-K4Ω ParaBAD-xcpT-phoAThis study
pUC19Apr; cloning vector55
pUX4pUC19 xcpTThis study
pSPT19Apr; cloning vectorPharmacia
pSX4pSPT19 xcpTThis study
pT7-6Apr; cloning vector; T7 Φ10 promoter49
pT7-TpT7-6 PΦ10-xcpTThis study
pGP1.2Kmr; T7 polymerase gene49
pUP2pUC19 xcpA12
pGP1.2ApGP1.2 xcpAThis study
pUR292Apr; cloning vector; promoterless lacZ41
pJGA03pYRC xcpT-lacZThis study
  • a Gm, gentamicin; Ap, ampicillin; Km, kanamycin; ss, signal sequence.