TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotype and relevant characteristicsSource or reference
Strains
    V. anguillarum 775(pJM1)Wild type 12
    V. anguillarum MS570 V. anguillarum 775 ttpC::Tn10Kmr TonB1::Cmr 36
    V. anguillarum MS801 V. anguillarum 775 tonB2::Kmr 36
    V. anguillarum MS995 V. anguillarum 775 ttpC::Tpr This study
    V. cholerae CA401Wild type 4
    V. cholerae DOV221 V. cholerae ΔexbB2 in strain CA401 26
    V. cholerae DOV300 V. cholerae ΔexbB2 ΔexbB1 in strain CA401 26
    V. cholerae MS1033 V. cholerae ttpC mutant in strain CA401This study
    E. coli HB101 supE44 hsd20 (rB mB ) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 8
    E. coli JM109λpir recA1 endA1 gyrA96 thi hsdR17 supE4relA1Δ(lac-proAB +) (F′ traD36 proAB lacI q lacZΔM15) λpir Lab collection
    E. coli W3110F IN(rrnD-rrnE)1 19
    E. coli KP1032 tonB mutant of E. coli W3110 23
Plasmids
    pJM1Indigenous plasmid in strain 775 12
    pRK2073Helper plasmid for conjugation; Tpr Tra+ 16
    pCR2.1-TOPOCloning vector; Kmr Apr Invitrogen
    pACYC177Cloning vector; Kmr Apr 10
    pTW-MEVSuicide vector, R6K ori, sacB; Apr 39
    pDM4Suicide vector R6K ori, sacB; Cmr 25
    p34E-TpVector harboring the Tpr cassette 14
    pUC4KVector harboring the Kmr cassette 37
    pCR8Cloning vector; Spr Invitrogen
    pMS989 V. anguillarum ΔttpC::Tpr in pDM4This study
    pMS1029 V. cholerae ttpC::Kmr in SalI in pTW-MEVThis study
    pMS789pACYC177 pKmr exbB2 exbD2 tonB2 36
    pMS800pACYC177 pKmr ttpC exbB2 exbD2 tonB2 This study
    pMS800-ΔexbB2 pMS800 with internal deletion in exbB2 This study
    pMS800-ΔexbD2 pMS800 with internal deletion in exbD2 This study