TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotypeaSource, reference, or construction
Strains
    MC1061E. coli K-12 araD Δ(ara-leu)7697 Δ(codB-lacI) galK16 galE15 mcrA0 relA1 rpsL150 spoT1 mcrB9999 hsdR2E. coli Genetic Stock Center (7)
    MC4100E. coli K-12 araD139 Δ(argF-lac)169 rpsL150 thiA1 relA1 flb5301 deoC1 ptsF25 rbsRE. coli Genetic Stock Center (6)
    MG1655E. coli K-12 (MG1655) rph-1E. coli Genetic Stock Center (16, 17)
    BB7063W3110 zad::Tn10 PA1/lacO-1-dnaKJ46
    TX2817MG1655 hfq2::Ω (Km; KpnI)48, 49
    TX2821MG1655 hfq1::Ω (Km; BclI)48, 49
    CAG2322MC4100 [Φ λrpoH′(MluI)-lacZ]Lab stocks
    CAG2323MC4100 [Φ λrpoH′(PvuII)-lacZ]Lab stocks
    CAG22357MC1061 [Φ λrpoH P3::lacZ] ΔrseA nadB::Tn10; Tetr3
    CAG25195MG1655 ΔlacX74 [Φ λrpoH P3::lacZ]37
    CAG25196MG1655 ΔlacX74 [Φ λrpoH P3::lacZ] pTrc99A37
    CAG25197MG1655 ΔlacX74 [Φ λrpoH P3::lacZ] pLC24537
    CAG25198MG1655 nadB::Tn10 ΔrseA ΔlacX74 [Φ λrpoH P3::lacZ]CAG25195 + P1/CAG22357
    CAG45146MG1655 ΔlacX74 [Φ λhtpG P1::lacZ]31
    CAG48383MG1655 ΩlacX74 [Φ λhtpG P1::lacZ] hfq2::Ω (Km; KpnI)CAG45146 + P1/TX2817
    CAG48400MG1655 ΩlacX74 [Φ λhtpG P1::lacZ] hfq2::Ω (Km; KpnI) PA1/lacO-1-dnaKJCAG48383 + P1/BB7063
    CAG48401MG1655 ΩlacX74 [Φ λhtpG P1::lacZ] hfq1::Ω (Km; BclI) PA1/lacO-1-dnaKJCAG48422 + P1/BB7063
    CAG48402MG1655 ΩlacX74 [Φ λhtpG P1::lacZ] hfq2::Ω (Km; KpnI) pGro7This study
    CAG48403MG1655 ΩlacX74 [Φ λhtpG P1::lacZ] hfq1::Ω (Km; BclI) pGro7This study
    CAG48408MC4100 hfq2::Ω (Km; KpnI) [Φ λrpoH′(MluI)-lacZ]CAG2322 + P1/TX2817
    CAG48409MC4100 hfq1::Ω (Km; BclI) [Φ λrpoH′(MluI)-lacZ]CAG2322 + P1/TX2821
    CAG48410MC4100 hfq2::Ω (Km; KpnI) [Φ λrpoH′(PvuII)-lacZ]CAG2323 + P1/TX2817
    CAG48411MC4100 hfq1::Ω (Km; KpnI) [Φ λrpoH′(PvuII)-lacZ]CAG2323 + P1/TX2821
    CAG48422MG1655 ΔlacX74 [Φ λhtpG P1::lacZ] hfq1::Ω (Km; BclI)CAG45146 + P1/TX2821
    CAG50008MG1655 ΔlacX74 [Φ λrpoH P3::lacZ] hfq2::Ω (Km; KpnI)CAG25195 + P1/TX2817
    CAG50009MG1655 ΔlacX74 [Φ λrpoH P3::lacZ] hfq1::Ω (Km; BclI)CAG25195 + P1/TX2821
    CAG50010MG1655 ΔlacX74 [Φ λrpoH P3::lacZ] hfq2::Ω (Km; KpnI), pTrc99AThis study
    CAG50011MG1655 ΔlacX74 [Φ λrpoH P3::lacZ] hfq2::Ω (Km; KpnI), pLC245This study
    CAG50013MG1655 ΔlacX74 [Φ λrpoH P3::lacZ] hfq1::Ω (Km; BclI), pLC245This study
Plasmids
    pGro7Vector, pACYC184 ori; Cmr; ParaB-groELS. Carries groELS induced from PBAD promoter30
    pTrc99AVector, pBR322 ori; Apr; expression vector containing an IPTG inducible trc promoterAmersham Pharmacia Biotech
    pLC245rpoE cloned in pTrc99A downstream of the IPTG inducible trc promoter; Apr37
  • a Cmr, chloramphenicol resistance; Tetr, tetracycline resistance; Apr, ampicillin resistance.