TABLE 3.

Kinetic constants for purified recombinant PduL-His8a

SubstrateKm (mM)Vmax (μmol min−1 mg−1)kcat (s−1)kcat/Km (mM s−1)
Propionyl-PO42−0.61 ± 0.0651.7 ± 7.620.733.9
Acetyl-PO42−0.97 ± 0.2613.4 ± 1.85.45.6
  • a PTAC assays were performed by monitoring the absorbance of reaction mixtures at 232 nm as described in Materials and Methods. The kinetic constants for propionyl-PO42− and acetyl-PO42− were determined using an excess of HS-CoA (0.4 mM).