TABLE 1.

Bacterial strains and plasmids used in this work

Strain or plasmidRelevant characteristicsaReference or source
E. coli JM109 recA 1 endA 1 gyrA 96 thi supE 44 relA 1 Δ(lac-proAB)/F′ [traD 36 lacIq lacZ ΔM15] 67
X. campestris pv. campestris strains
    8004Wild type; Rifr
    8004*8004 derivative containing an E45K conversion in HrpG; Rifr 15
    8004* ΔhrpX As 8004* but hrpX deleted; Rifr Kanr 27
    NK2827As 8004 but hpaR::pK18mob; Rifr Kanr 27
    NK2827*As 8004* but hpaR::pK18mob; Rifr Kanr This work
    CNK2827NK2827 harboring pXC2827; Rifr Kanr Tcr This work
    8004G8004 ΔhrpG; Rifr Kanr This work
    8004X8004 ΔhrpX; Rifr Kanr Laboratory collection
    8004pG288004 harboring pGUS2827; Rifr Kanr Tcr Laboratory collection
    8004GpG288004G harboring pGUS2827; Rifr Kanr Tcr This work
    8004XpG288004X harboring pGUS2827; Rifr Kanr Tcr This work
    8004RpG28NK2827 harboring pGUS2827; Rifr Kanr Tcr This work
    161E12As 8004 but XC_2826::Tn5gusA5; Rifr Kanr Spcr Gmr This work
    NK2828As 8004 but XC_2828::pK18mob; Rifr Kanr Laboratory collection
Plasmids
    pLAFR6Broad-host-range cloning vector; Tcr 23
    pRK2073Helper plasmid; Tra+ Mob+ ColE1 Spcr 30
    pK18mobSuicide plasmid in X. campestris pv. campestris; Kanr 45
    pT18mobTetracycline-resistant derivative of pK18mob; Tcr Laboratory collection
    pK2827pK18mob containing a 267-bp internal fragment of the hpaR gene; Kanr This work
    pT2827pT18mob containing a 267-bp internal fragment of the hpaR gene; Kanr This work
    pXC2827pLAFR6 containing a 758-bp fragment including the hpaR gene; Tcr This work
    pGUS2827pLAFR6 containing a hpaR promoter-gusA fusion fragment; Tcr This work
    pGUShrpA pLAFR6 containing a hrpA operon promoter-gusA fusion fragment; Tcr This work
    pGUShrpB pLAFR6 containing a hrpB operon promoter-gusA fusion fragment; Tcr Laboratory collection
    pGUShrpC pLAFR6 containing a hrpC operon promoter-gusA fusion fragment; Tcr Laboratory collection
    pGUShrpD pLAFR6 containing a hrpD operon promoter-gusA fusion fragment; Tcr Laboratory collection
    pGUShrpE pLAFR6 containing a hrpE operon promoter-gusA fusion fragment; Tcr Laboratory collection
    pGUShrpF pLAFR6 containing a hrpF operon promoter-gusA fusion fragment; Tcr Laboratory collection
    pGUShrpG pLAFR6 containing a hrpG promoter-gusA fusion fragment; Tcr Laboratory collection
    pGUShrpX pLAFR6 containing a hrpX promoter-gusA fusion fragment; Tcr Laboratory collection
  • a Rifr, Kanr, Spcr, Gmr, and Tcr, rifampin, kanamycin, spectinomycin, gentamicin, and tetracycline resistant, respectively.