TABLE 3.

Genes differentially expressed in JAM1a

Unique ID (GenBank)DescriptionMicroarray difference (n-fold) of geom means JAM1/UA159Microarray parametric P valueReal-time PCR fold difference of geom means JAM1/UA159Real-time PCR parametric P valueReal-time PCR primers
SMU.1596Putative PTS system, cellobiose-specific IIC component132.429P < 1E−071,111.110.002F-GGCTGGGCAATGAGTAATGG, R-TGACAAAACCAGGGATTAAAGC
SMU.1601Putative phospho-beta-glucosidase BglA100.6115.83E−05
SMU.148Putative alcohol-acetaldehyde dehydrogenase35.1463.00E−0715.40.031F-AACGGTTGGCATCATTGGTG, R-TGTTGGGTTAGTTGTTGGTACG
SMU.1598Putative PTS system, cellobiose-specific IIA component26.1140.0021912474.720.0087F-GAATTACAAGTTGCCGCATTTG, R-TCTGTGCTTGATGAGCTTTGAG
SMU.1411Conserved hypothetical protein23.543.40E−06
SMU.936Putative amino acid ABC transporter, ATP-binding protein18.1479.41E−05
SMU.962Putative dehydrogenase13.4520.000102
SMU.934Putative amino acid ABC transporter, permease protein12.950.00051413.60.000062F-AGAAGCTGGTCTCAGTATTGG, R-ACCGATTGTATAGGCTAGAGC
SMU.1536Putative starch (bacterial glycogen) synthase12.2834.70E−06
SMU.935Putative amino acid ABC transporter, permease protein10.910.000406
SMU.114Putative PTS system, fructose-specific IIBC component10.5290.00226567.00.0067F-AGCCGACAAGGATGTTCAAGC, R-CTTTGCCAGCTAAAGCATCTGC
SMU.1537Putative glycogen biosynthesis protein GlgD10.4071.95E−0515.30.0046F-GCTATCGGATTCCCAGAAATGG, R-CACGACCGCTTCTGATATGATC
SMU.933Putative amino acid ABC transporter, periplasmic amino10.220.0008668
SMU.496Putative cysteine synthetase A O-acetylserine lyase9.6683.22E−05
SMU.961Conserved hypothetical protein9.1910.00084014.60.0048F-AACGGCTCTTGAGACTTATCG, R-TCTGGTGCCATTTGAATTTGC
SMU.180Putative oxidoreductase possible fumarate reductase8.9480.0001778
SMU.402Pyruvate formate-lyase8.6551.03E−0511.50.019F-AAGGAACTGACTGGAAAGAC, R-CGTGTTTCTTCGTAATGCG
SMU.2127Putative succinate semialdehyde dehydrogenase7.8950.0017855
SMU.149Putative transposase7.8660.0017717
SMU.1538Putative glucose-1-phosphate adenylyltransferase ADP-glucose pyrophosphorylase7.4446.80E−06
SMU.1535Glycogen phosphorylase7.1299.36E−05
SMU.312PTS system, sorbitol phosphotransferase enzyme IIBC6.3264.50E−06100.07F-TCGCTCTGGCTATTGTTGACTG, R-TGGGCTAAAGGTCCGCTCTTAC
SMU.1077Putative phosphoglucomutase5.5151.37E−054.10.03F-ATTGGCGCTGGAACTAATCG, R-TTGAGAGAAATGACGGGAATCG
SMU.527Conserved hypothetical protein4.9882.79E−05
SMU.616Hypothetical protein4.8426.19E−05
SMU.1422Putative pyruvate dehydrogenase E1 component beta subunit4.8350.0028314
SMU.179Conserved hypothetical protein4.7620.00056584.550.012F-ATGATTGTGGGCTGTTCG, R-CGTGTTCAGAGTGACCTAG
SMU.1603Putative lactoylglutathione lyase4.5330.0022774
SMU.508Conserved hypothetical protein4.4980.0028192
SMU.1013cPutative Mg2+/citrate transporter4.460.0009883
SMU.308Sorbitol-6-phosphate 2-dehydrogenase4.1960.0010415
SMU.1187Glucosamine-fructose-6-phosphate aminotransferase4.0375.20E−05
SMU.1254Conserved hypothetical protein40.0022115
SMU.360Extracellular glyceraldehyde-3-phosphate dehydrogenase3.9730.0011203
SMU.675Phosphoenolpyruvate:sugar phosphotransferase system enzyme I, PTS system3.8138.10E−061.60.014F-GCAGTAGATACCCTTGGTGAAG, R-TCTGTCACTTCTTTGAGAGCAC
SMU.460Putative amino acid ABC transporter, permease3.7230.0001489
SMU.426Copper-transporting ATPase P-type ATPase3.2710.0007648
SMU.882Multiple sugar-binding ABC transporter, ATP-binding protein, MsmK3.1610.0006518
SMU.361Phosphoglycerate kinase3.1410.0001563
SMU.674Phosphoenolpyruvate:sugar phosphotransferase system HPr3.0660.00076861.70.01F-CATGCACGCCCAGCTACTTTG, R-CATCAGCACCTTGACCAACACC
SMU.1247Putative enolase2.8510.00015290.480.07F-TGGTTCCTTCAGGAGCTTCTAC, R-CCGTCAAGTGCGATCATTGC
SMU.509Conserved hypothetical protein2.8450.00060420.730.2F-GTTGGTCAGGTTCGACAAGC, R-TGGATACTTGGCAAAAGGATGG
SMU.1943Putative leucyl-tRNA synthetase2.6430.0019886
SMU.859Putative carbamoyl phosphate synthetase, small subunit2.3730.0024238
SMU.1115Lactate dehydrogenase2.3320.000617
SMU.1563Putative cation-transporting P-type ATPase PacL2.1350.0014719
SMU.1856cConserved hypothetical protein0.4150.0015245
SMU.613Hypothetical protein0.3610.0018835
SMU.1009Putative histidine kinase0.3010.0007651
SMU.2097Hypothetical protein0.2950.0023821
SMU.1516Putative histidine kinase CovS VicK homolog0.2840.00045490.550.017F-CCTTAAACCGCCGTGAAAGTGG, R-AGGGCACCATCGTCCAAAGC
SMU.1432cPutative endoglucanase precursor0.2790.000974
SMU.131Putative lipoate-protein ligase0.2590.00074
SMU.128Putative acetoin dehydrogenase (TPP-dependent), E1 component beta0.2560.0015098
SMU.1744Putative 3-oxoacyl-(acyl-carrier-protein) synthase III0.2530.00138060.520.014F-AGTTATCGGTGCAGAAGTTC, R-AAAGGGTGACGAAACAGC
SMU.2042Dextranase precursor0.2086.26E−050.230.003F-TTATTCCTGCAAACTCCTTAGC, R-ACCTCCAATAGCAGCATAACG
SMU.872Putative PTS system, fructose-specific enzyme IIABC component0.1810.00036320.110.045F-TTAAGGCTGGGATCATGAATCG, R-AACAGGTTCACCATCAAGAGC
SMU.1926Putative transcriptional regulator0.1770.0026323
SMU.2038Putative PTS system, trehalose-specific IIABC component0.1610.00037890.120.005F-GTATTGAAGGGGTCTCTAAGG, R-ATTACGAAATCCAAGAATCAGC
SMU.602Putative sodium-dependent transporter0.1570.002202
SMU.870Putative transcriptional regulator of sugar metabolism0.1310.0007458
SMU.871Putative fructose-1-phosphate kinase0.1080.0004296
  • a ID, identification number; geom, geometric; F, forward; R, reverse.