TABLE 2.

Selected P. aeruginosa genes that are regulated by growth in the presence of a limiting concentration of Mg2+ in wild-type (H103), phoP::xylE (H851), and pmrA::xylE (H988) strains sorted by categorya

PA no.Gene designationComparison of growth
H103 with low Mg2+ concn vs H103 with high Mg2+ concnH851 with low Mg2+ concn vs H103 with high Mg2+ concnH988 with low Mg2+ concn vs H103 with high Mg2+ concn
Fold changeP valueFold changeP valueFold changeP value
Positively Mg2+ regulated
    PA0762balgU4.10.041.9<0.012.6<0.01
    PA0763bmucA3.4<0.012.30.081.80.05
    PA0764bmucB2.5<0.011.90.014.20.02
    PA12973.3<0.013.1<0.013.0<0.01
    PA3552pmrH7.2<0.015.6<0.016.9<0.01
    PA3553pmrF12.5<0.015.30.057.60.05
    PA3554pmrI11.9<0.0110.4<0.0111.6<0.01
    PA3555pmrJ3.8<0.013.0<0.013.7<0.01
    PA3556pmrK, arnT10.6<0.019.6<0.017.8<0.01
    PA3557pmrL3.7<0.011.60.63−1.30.37
    PA3558pmrM3.60.012.0<0.011.80.01
    PA3559ugd8.0<0.015.3<0.013.9<0.01
    PA39205.4<0.018.60.016.2<0.01
Negatively Mg2+ regulated
    PA2260kguE−3.30.03−1.9<0.01−2.9<0.01
    PA2261kguK−5.4<0.01−6.7<0.01−7.8<0.01
    PA2262kguT−3.40.01−2.6<0.01−3.3<0.01
    PA2265gad−2.8<0.01−2.20.04−2.10.01
    PA2266−2.7<0.01−2.3<0.01−2.6<0.01
    PA2321−5.20.01−3.2<0.01−4.2<0.01
    PA2322gntT−16.9<0.01−16.4<0.01−9.5<0.01
    PA2323−11.1<0.01−9.2<0.01−10.7<0.01
    PA4236katA−2.50.05−2.7<0.01−3.1<0.01
    PA4463−6.6<0.01−4.70.01−4.6<0.01
    PA4761dnaK−2.90.02−2.80.03−1.8<0.01
    PA4835−2.20.01−2.4<0.01−2.4<0.01
    PA4836−2.10.02−4.6<0.01−3.0<0.01
Positively Mg2+ and positively PhoP regulated
    PA0265gabD5.2<0.011.40.064.00.01
    PA0266gabT5.1<0.011.20.094.00.05
    PA09212.2<0.01−1.10.572.6<0.01
    PA1178oprH7.6<0.01−1.80.0118.40.03
    PA1179bphoP19.8<0.01−1.20.2939.8<0.01
    PA1180bphoQ5.9<0.01−1.20.098.0<0.01
    PA3522mexQ16.4<0.013.1<0.017.9<0.01
    PA36492.5<0.01−1.80.802.3<0.01
    PA3885b2.70.05−1.50.051.4<0.01
    PA40107.0<0.01−1.10.084.7<0.01
    PA40112.00.01−1.10.472.3<0.01
    PA44532.8<0.01−1.30.044.9<0.01
    PA44542.2<0.01−1.30.073.1<0.01
    PA44552.6<0.01−1.20.081.8<0.01
Negatively Mg2+ and negatively PhoP regulated
    PA0836back−4.0<0.01−1.50.04−5.7<0.01
    PA1196b−3.1<0.01−1.30.07−4.2<0.01
    PA3309−3.2<0.011.60.14−8.6<0.01
    PA4918−2.50.02−1.40.85−2.8<0.01
Not Mg2+ regulated and positively PhoP regulated
    PA4366sodB−1.30.26−5.2<0.01−1.10.49
Positively Mg2+ regulated and positively PmrA regulated
    PA02012.60.032.8<0.01−2.40.08
    PA02022.2<0.011.10.12−1.20.26
    PA0282cysT3.60.012.7<0.01−1.20.02
    PA0546metK2.7<0.011.80.06−1.00.60
    PA0913mgtE2.80.012.7<0.01−1.20.61
    PA15594.00.0113.6<0.012.4<0.01
    PA15603.2<0.018.0<0.012.9<0.01
    PA2359b3.40.011.7<0.011.00.51
    PA34462.30.022.80.01−1.60.06
    PA43574.60.031.40.03−1.30.06
    PA4358feoB3.3<0.015.4<0.01−1.70.03
    PA4359feoA4.0<0.012.20.10−2.30.01
    PA47734.1<0.013.20.041.00.45
    PA47746.20.0219.0<0.011.00.70
    PA47751.00.221.10.081.00.25
    PA4776bpmrA3.3<0.014.5<0.01−1.30.05
    PA4777bpmrB6.7<0.022.8<0.01−1.10.49
    PA4778b4.4<0.012.7<0.011.10.02
    PA4781b2.2<0.012.0<0.011.10.67
    PA478212.7<0.016.2<0.01−1.10.02
    PA48227.7<0.016.9<0.01−1.9<0.01
    PA48235.8<0.019.0<0.01−1.00.69
    PA48248.1<0.0113.3<0.01−1.10.02
    PA4825mgtA10.0<0.0130.5<0.01−2.4<0.01
    PA48265.2<0.013.60.02−1.40.03
Negatively Mg2+ regulated and positively PmrA regulated
    PA0527bdnr−3.10.01−1.70.01−9.8<0.01
Positively Mg2+ regulated and negatively PmrA regulated
    PA2064pcoB3.20.012.00.044.4<0.01
    PA2065pcoA3.20.022.00.0118.6<0.01
    PA35156.1<0.017.30.02150.0<0.01
    PA35163.60.011.30.165.90.02
    PA351713.7<0.0111.2<0.0122.1<0.01
    PA35188.0<0.017.20.0155.5<0.01
Not Mg2+ regulated and negatively PmrA regulated
    PA22741.20.181.50.215.4<0.01
    PA4205mexG1.40.051.20.166.5<0.01
    PA4206mexH1.30.061.30.055.0<0.01
    PA4207mexI1.20.171.20.192.3<0.01
  • a Only selected Mg2+ genes that were not affected by phoP::xylE or pmrA::xylE mutations are included. All results were standardized to expression of the wild-type strain in the fully repressed state (i.e., in the presence of 2 mM Mg2+).

  • b Known or putative regulatory gene.