TABLE 4.

PmrA-like promoters identified by consensus sequence searching

Sequence or operonSequenceaGenome coordinatesMg2+ regulatedbPmrA regulatedbGel shift
Consensus sequenceNTTAASNNNNNCTTAAS
pmrH-ugdGTTAAGCGAGTCTTAAC3979769-3979785YesYesYesc
ATTAATGAACCCTTAAA3979614-3979630
PA4773-pmrABGTTAAGCACCTCTTAAG5361502-5361518YesYesYes
feoAB-PA4357CTTAAGCGAGCCTTAAG4887593-4887577YesYesYes
PA1559-PA1560ATTAACGGTTCCTTAAG1697095-1697111YesYesYes
PA4782-PA4781CTTAAGCGATCCCTAAG5370750-5370734YesYesNDe
PA0327TTTAAGCTTGGCTTCAG367370-367386NoNTdNT
algD-8-44-KEGXLIGFACTTAAGGTTTGCTTAAG3962241-3962257NoNTNT
PA0545GTTAAGGGACAATTAAG602272-602288NoNTNT
PA0053TTTAAGCATGTCGCAAG69049-69065NoNTNT
aprA (PA1249)TTTAAGTGCAGCTTAAT1355477-1355493NoNTNT
PA2050-PA2051TTTAAGTGCAGCTTAAT1355477-1355493NoNTNT
PA4500TTTCAGCATGACTTAAT5036896-5036912NoNTNT
PA2505-PA2506ATTAAGTGCGAGTTAAG2824124-2824108NoNTNT
PA3868ATTAAGGCCTGCTCAAC4331298-4331314NoNTNT
PA4498-PA4499TTTAAGCTCGACTTAAA5036126-5036110NoNTNT
PA5106TTTACGCACCGCTTAAT5749388-5749404NoNTNT
  • a Nucleotides that differ from the nucleotides in the consensus sequence are indicated by boldface type. Abbreviations: N = A, C, G, or T; S = C or G.

  • b Determined by reverse transcription-qPCR.

  • c Promoter regions were tested for binding to PhoP and PmrA. Only the pmrH-ugd promoter fragment bound to both. The other promoters bound only to PmrA and not to PhoP.

  • d NT, not tested (no Mg2+ regulation could be demonstrated, and no regulation was observed in array experiments).

  • e ND, not determined.