TABLE 4.

PmrA-like promoters identified by consensus sequence searching

Sequence or operonSequenceaGenome coordinatesMg2+ regulatedbPmrA regulatedbGel shift
Consensus sequence NTTAASNNNNNCTTAAS
pmrH-ugd GTTAAGCGAGTCTTAAC 3979769-3979785YesYesYesc
ATTAATGAACCCTTAAA 3979614-3979630
PA4773-pmrAB GTTAAGCACCTCTTAAG 5361502-5361518YesYesYes
feoAB-PA4357 CTTAAGCGAGCCTTAAG 4887593-4887577YesYesYes
PA1559-PA1560 ATTAACGGTTCCTTAAG 1697095-1697111YesYesYes
PA4782-PA4781 CTTAAGCGATCCCTAAG 5370750-5370734YesYesNDe
PA0327 TTTAAGCTTGGCTTCAG 367370-367386NoNTd NT
algD-8-44-KEGXLIGFA CTTAAGGTTTGCTTAAG 3962241-3962257NoNTNT
PA0545 GTTAAGGGACAATTAAG 602272-602288NoNTNT
PA0053 TTTAAGCATGTCGCAAG 69049-69065NoNTNT
aprA (PA1249) TTTAAGTGCAGCTTAAT 1355477-1355493NoNTNT
PA2050-PA2051 TTTAAGTGCAGCTTAAT 1355477-1355493NoNTNT
PA4500 TTTCAGCATGACTTAAT 5036896-5036912NoNTNT
PA2505-PA2506 ATTAAGTGCGAGTTAAG 2824124-2824108NoNTNT
PA3868 ATTAAGGCCTGCTCAAC 4331298-4331314NoNTNT
PA4498-PA4499 TTTAAGCTCGACTTAAA 5036126-5036110NoNTNT
PA5106 TTTACGCACCGCTTAAT 5749388-5749404NoNTNT
  • a Nucleotides that differ from the nucleotides in the consensus sequence are indicated by boldface type. Abbreviations: N = A, C, G, or T; S = C or G.

  • b Determined by reverse transcription-qPCR.

  • c Promoter regions were tested for binding to PhoP and PmrA. Only the pmrH-ugd promoter fragment bound to both. The other promoters bound only to PmrA and not to PhoP.

  • d NT, not tested (no Mg2+ regulation could be demonstrated, and no regulation was observed in array experiments).

  • e ND, not determined.