TABLE 3.

Decrease of hpa1 expression in the trh-defective mutant during growth in rice leaves and pathogenicity of the mutants

StrainaBioluminescence (cpm)b,cNo. of bacteria (107 CFU)c,dLesion length (mm)c,e
74Hpa1::Lux4,802.8 ± 1,521.34.7 ± 3.240.0 ± 7.8
74Hpa1::Lux/Trh::Kan910.0 ± 434.04.0 ± 2.237.8 ± 9.2
  • a Each bacterium was used to inoculate the susceptible rice cultivar IR24.

  • b Bioluminescence from 1-cm-long leaf sections, including the inoculation site, was measured 3 days after inoculation, and photons per minute are shown.

  • c Values are averages ± standard deviations for five inoculated leaves.

  • d Bacterial numbers from 1-cm-long leaf sections, including the inoculation site, were measured by plating on the medium 3 days after inoculation.

  • e Measured 2 weeks after inoculation.