TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant propertiesSource or reference
E. coli strains
    DH5αF′ endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 (φ80lacΔM15)Promega
    CC118 (λ pir)Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λ pir26
    S17-1 (λ pir)Tpr Smr recA thi pro rK mK+ RP4:2-Tc:MuKm Tn7 λ pir16
V. cholerae strains
    FY_Vc_2O1 El Tor A1552, rugose variant, Rifr69
    FY_Vc_1040rbmB, FY_Vc_2 ΔrbmB, RifrThis study
    FY_Vc_686rbmC, FY_Vc_2 ΔrbmC, RifrThis study
    FY_Vc_690rbmD, FY_Vc_2 ΔrbmD, RifrThis study
    FY_Vc_742rbmEF, FY_Vc_2 ΔrbmEF, RifrThis study
    FY_Vc_1367bap1, FY_Vc_2 Δbap1, RifrThis study
    FY_Vc_1400rbmC Δbap1, FY_Vc_1367 ΔrbmC, RifrThis study
    FY_Vc_222FY_Vc_2 mTn7-GFP, Gmr20
    FY_Vc_1318FY_Vc_1040 mTn7-GFP, GmrThis study
    FY_Vc_1321FY_Vc_686 mTn7-GFP, GmrThis study
    FY_Vc_1387FY_Vc_690 mTn7-GFP, GmrThis study
    FY_Vc_1324FY_Vc_742 mTn7-GFP, GmrThis study
    FY_Vc_1392FY_Vc_1367 mTn7-GFP, GmrThis study
    FY_Vc_1395FY_Vc_1400 mTn7-GFP, GmrThis study
Plasmids
    pGP704-sacB28pGP704 derivative, mob/oriT sacB, AprG. Schoolnik
    pFY-271pGP704-sacB28::ΔrbmB, AprThis study
    pFY-272pGP704-sacB28::ΔrbmC, AprThis study
    pFY-273pGP704-sacB28::ΔrbmD, AprThis study
    pFY-274pGP704-sacB28::ΔrbmEF, AprThis study
    pFY-330pGP704-sacB28::Δbap1, AprThis study
    pACYC177Multicopy cloning vector, AprNew England Biolabs
    pFY-361prbmB, pACYC177::rbmB, AprThis study
    pFY-362prbmC, pACYC177::rbmC, AprThis study
    pFY-363prbmD, pACYC177::rbmD, AprThis study
    pFY-364prbmEF, pACYC177::rbmEF operon, AprThis study
    pFY-366pbap1, pACYC177::bap1, AprThis study
    pBAD/Myc-His BArabinose-inducible expression vector with C-terminal Myc epitope and six His codons, AprInvitrogen
    pFY-417prbmB-myc, pBAD/Myc-His B::rbmB coding region, AprThis study
    pFY-420prbmC-myc, pBAD/Myc-His B::rbmC coding region, AprThis study
    pFY-429pbap1-myc, pBAD/Myc-His B::bap1 coding region, AprThis study
    pvpsA::lacZpRS415 vpsA promoter, Apr13
    pvpsL::lacZpRS415 vpsL promoter, Apr13
    pMCM11pGP704::mTn7-GFP, Gmr AprG. Schoolnik
    pUX-BF13oriR6K helper plasmid, mob/oriT, provides Tn7 transposition function in trans, Apr6