TABLE 3.

COMSTAT quantitative analysis of biofilm structures formed by V. cholerae rugose variant and deletion mutants of rbmB-F and bap1a

StrainTotal biomass (μm3/μm2)Thickness (μm)Substrate coverageRoughness coefficient
AvgMaximum
Rugose15.6 (3.3)17.5 (4.9)56.7 (15.1)1.0 (0.01)0.5 (0.1)
rbmB15.7 (3.3)18.2 (5.4)65.6 (12.5)1.0 (0.02)0.7 (0.1)
rbmC13.7 (1.8)15.0 (2.0)52.8 (2.1)1.0 (0.01)0.6 (0.1)
bap116.9 (2.9)19.2 (3.7)54.3 (9.7)0.9 (0.1)0.5 (0.04)
rbmC Δbap10.4 (0.3)0.5 (0.3)20.9 (5.8)0.1 (0.04)1.9 (0.1)
rbmD15.7 (2.3)20.4 (3.4)58.3 (11.1)1.0 (0.02)0.4 (0.1)
rbmEF16.1 (1.0)17.3 (1.2)53.0 (1.9)1.0 (0.001)0.3 (0.04)
  • a Values presented are means of data from at least five z-series image stacks taken at 24 h. The numbers in parentheses indicate standard deviations.