TABLE 3.

Physiological effects of S. putrefaciens CN-32 with cymA heme variants on the reduction of arsenatea

StrainTime (h)Arsenate (mM)Arsenite (mM)
All09.9 ± 0.220.23 ± 0.32
CN-32 + pBBR1MCS-212.50.7 ± 0.119.7 ± 0.00
50.509.7 ± 0.06
CN-CYMA + pBBR1MCS-212.59.7 ± 0.140.12 ± 0.16
50.51.4 ± 0.218.6 ± 0.01
CN-CYMA + pH 1(C46S)12.59.2 ± 0.301.0 ± 0.27
27.5010.2 ± 0.50
50.509.8 ± 0.26
CN-CYMA + pH 2(C78S)12.510.1 ± 0.120.10 ± 0.12
50.51.5 ± 0.218.1 ± 0.13
CN-CYMA + pH 3(C136S)12.59.8 ± 0.030.18 ± 0.16
50.52.5 ± 0.367.2 ± 0.11
CN-CYMA + pH 4(C173S)12.59.9 ± 0.220.07 ± 0.05
50.51.4 ± 1.989.9 ± 0.61
  • a The cultures represented in Fig. 6B were sampled at the indicated times, and the filtrates were analyzed by high-performance liquid chromatography for arsenate and arsenite. The data and errors represent the means and standard deviations of triplicate cultures, respectively.