TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or markers, characteristics, and usesSource or reference
E. coli strains
    TOPO Top 10E. coli host for cloning; F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
    UQ950E. coli DH5α λ(pir) host for cloning; F Δ(argF-lac)169 φ80dlacZ58(ΔM15) glnV44(AS) rfbD1 gyrA96(NalR) recA1 endA1 spoT1 thi-1 hsdR17 deoR λpir+28
    WM3064Donor strain for conjugation; thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir(wt)]28
Shewanella strains
    Shewanella sp. ANA-3Isolated from an As-treated wooden pier piling in a brackish estuary (Eel Pond, Woods Hole, MA)27
    AN-CYMAShewanella sp. strain ANA-3; ΔcymA; does not respire As(V) or fumarateThis study
    S. putrefaciens CN-32Isolated from anaerobic subsurface core sample, New Mexico8, 34
    CN-CYMAS. putrefaciens CN-32; ΔcymA; does not respire As(V)This study
    Shewanella sp. W3-18-1Isolated from Pacific Ocean marine sediments at 630 m19
Plasmids/vectors
    pCR4-TOPO4-kb cloning vector; lacZ-ccdB: Kmr AmprInvitrogen
    pSMV109.1-kb mobilizable suicide vector; oriR6K mobRP4 sacB Kmr Gmr28
    ANpΔcymA1.2-kb fusion PCR fragment containing ΔcymA from ANA-3 cloned into the SpeI site of pSMV10; used to make the AN-CymA ΔcymA strain.This study
    CNpΔcymA2-kb fusion PCR fragment containing ΔcymA from S. putrefaciens cloned into the SpeI site of pSMV10; used to make the CN-CymA ΔcymA strain.This study
    pBBR1MCS-25.1-kb broad-host-range plasmid; Kmr lacZ14
    pANcymAANA-3 cymA PCR fragment, including the promoter region, cloned into the SpeI site of pBBR1MCS-2This study
    pCNcymAS. putrefaciens cymA PCR fragment cloned into the SpeI sites of pBBR1MCS-2This study
    pH 1(C46S)pCNcymA plasmid with SXXCH of heme 1This study
    pH 2(C78S)pCNcymA plasmid with CXXSH of heme 2This study
    pH 3(C136S)pCNcymA plasmid with SXXCH of heme 3This study
    pH 4(C173S)pCNcymA plasmid with SXXCH of heme 4This study