TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)aSource
Strains
    E. coli
        TG1supE Δ(lac-proAB) thi hsdRΔ5 (F′ traD36 rpoA+ B+ lacIqZΔM15)Lab collection
        TOP10F′F′ [lacIq Tn10 (Tetr)] mrcA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1Invitrogen
        S17-1Conjugative helper strain; galU galK rpsL (Strr) endA1 nupG thi pro hsdR hsdM recA (RP4-2Tc::Mu Km::Tn7)λpirLab collection
        CC118(λpir)Host strain for pKNG101 replication; Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 Rfr (λpir)Lab collection
    P. aeruginosa
        PAO1Wild typeLab collection
        PAO1ΔflpPAO1 deletion mutant for flp geneThis study
        PAO1ΔtadAPAO1 deletion mutant for tadA geneThis study
        PAO1ΔrcpAPAO1 deletion mutant for rcpA geneThis study
        PAO1ΔfppAPAO1 deletion mutant for fppA geneThis study
        PAO1ΔpilDPAO1 deletion mutant for pilD geneLab collection
        PAO1ΔfliCΔpilAPAO1 deletion mutant for fliC and pilA genesLab collection
Plasmids
    pCR2.1TA cloning vector for PCR products; lacZα ColE1 f1 ori Apr KmrInvitrogen
    pMMB190Broad-host-range vector; IncQ ptac lacZα AprLab collection
    pBBR1MCS-2Broad-host-range vector; KmrLab collection
    pKNG101Suicide vector in P. aeruginosa; sacB StrrLab collection
    pMMBflpflp gene cloned in pMMB190 at BamHI/HindIIIThis study
    pBBRfppAfppA gene cloned in pBBR1MCS-2 at EcoRIThis study
    pBBRfppAD17AfppA gene with D17A substitution cloned into pBBR1MCS-2This study
    pBBRfppAD78AfppA gene with D78A substitution cloned into pBBR1MCS-2This study
    pBBRfppAD17AD78AfppA gene with D17A and D78A substitutions cloned into pBBR1MCS-2This study
    pDEST14Destination vector of the Gateway system, T7 terminator, T7 promoter; Cmr AprInvitrogen
    pLAFR3Cosmid, IncP, lac promoter; TcrLab collection
    pLAFRrcpArcpA gene cloned into pLAFR3 at BamHI/HindIIIThis study
    pLAFRtadAtadA gene cloned into pLAFR3 at BamHI/HindIIIThis study
    pPRK2013ColE1 ori tra+ mob+ KmrLab collection
  • a Strr, streptomycin resistance; Apr, ampicillin resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance.