TABLE 3.

Genes up-regulated in N. gonorrhoeae strain MS11-A after heat shock at 42°C for 10 min

Open reading frameaGeneFold changebProposed functioncRpoH regulond
Heat shock response, chaperones, and protein modification and degradation
    NGO1046 clpB 3.0HSP, chaperoneYes
    NGO0775 lon 2.4ATP-dependent proteaseYes
    NGO1422 grpE 2.4HSP, chaperoneYes
    NGO0383 ftsJ 1.9HSP, cell division protein
    NGO1189 hsp33 1.7Chaperone
    NGO0399 htpX 1.7HSP
    NGO1901 dnaJ 1.7HSP, chaperoneYes
Other
    NGO21422.4Hypothetical protein
    NGO12222.1OsmC-like family proteinYes
    NGO1244 marR 2.0Transcriptional regulator, MarRYes
    NGO0570 creA 2.0DNA-binding proteinYes
    NGO14261.9Conserved hypothetical proteinYes
    NGO0848 leuI 1.72-Isopropylmalate synthase
    NGO0679 leuC 1.73-Isopropylmalate dehydratase
    NGO0114 grxC 1.7Glutaredoxin 3
    NGO05681.7Hypothetical protein
    NGO14281.7Hypothetical protein
    NGO0999 rpoD 1.6RNA polymerase sigma factor
    NGO1694 folA 1.6Dihydrofolate reductaseYes
    NGO06781.6Hypothetical protein
    NGO12451.5Hypothetical protein
    NGO14251.8Hypothetical protein
    NGO03921.6Conserved hypothetical protein
    NGO18591.5Ferredoxin
  • a Designations from the annotation at www.ncbi.nlm.nih.gov/entrez/query.fcgi .

  • b The fold change is the ratio of the mRNA transcript level determined by microarray analysis in heat-stressed cells to the mRNA transcript level in nonstressed cells and is the average expression ratio for genes that were up-regulated at least 1.5-fold with a P value less than 0.001 in two biological repeats.

  • c Proposed functions of the genes based on sequence annotation, sequence homology, and previous investigations. HSP, heat shock protein

  • d Yes indicates that the gene is up-regulated as determined by microarray analysis with RpoH overexpression (Table 1).