TABLE 4.

Interactions among cag PAI-encoded proteins detected in a yeast two-hybrid systema

Interacting protein partnersbβ-Galactosidase activityc
GAL4AD-GAL4BD-
Cag3(B)Cag3(B)++
Cag3(B)CagT/12++
Cag3(B)CagM/16+
Cag3(B)CagG/21++
Cag5Cag5++
Cag5CagZ/6+
Cag5CagM/16+
Cag5CagI/19+
Cag5CagG/21+
VirB11/ATPaseVirB11/ATPase+++
VirB11/ATPaseCagY/7(B)++
CagZ/6CagS/13+
CagZ/6CagM/16+
CagZ/6CagI/19++
CagZ/6CagG/21++
CagZ/6*CagF/22*++
CagY/7(A)CagF/22+++
CagY/7(C)CagZ/6+
CagY/7(C)*CagX/8*+++
CagY/7(C)CagM/16+
CagX/8CagZ/6+
CagX18*CagY/7(C)+++
CagX/8*CagM/16*+
CagV/10Cag3(A)++
CagV/10CagZ/6+
CagV/10CagT/12++
CagV/10CagM/16+
CagT/12CagZ/6+
CagT/12*CagM/16*+
CagM/16*CagX/8*++
CagM/16*CagT/12*+
CagM/16CagS/13+
CagM/16CagM/16++
CagI/19CagT/12+
CagI/19CagG/21++
CagG/21CagT/12+
CagG/21CagM/16++
CagG/21CagF/22++
CagF/22*CagZ/6*+
CagF/22CagT/12+
CagF/22CagM/16+
CagE/23/PicBCagZ/6+
CagE/23/PicBCagY/7(C)+
CagE/23/PicBCagM/16+
  • a Cotransformation with the indicated plasmids resulted in the growth of yeast on plates lacking Trp, Leu, and His. β-Galactosidase activity is indicated in the right column.

  • b Asterisks indicate interactions that were detected with two reciprocal pairs of plasmids.

  • c +++ indicates highest activity, similar to the positive control; ++ indicates medium activity, similar to the mutant positive control; + indicates low activity, lower than the mutant positive control but higher than the negative control.