TABLE 1.

Strains used in this studya

StrainCharacteristicsP1 transduction or conjugationReference or source
CC101Derivative of strain P90C [araA(lac proB)xIII] carrying F′ lacIZ- proB+; lacZ has a mutation (GAG to TAG) at codon 4617
CC104Derivative of strain P90C [araA(lac proB)xIII] carrying F′ lacIZ- proB+; lacZ has a mutation (GAG to GCG) at codon 4617
AR30ΔdinB61::ble sulA2114
DE2302thr-1 ara-14 leuB6 Δ(gpt proA)62 lacY1 tsx-33 supE44 galK2 hisG4 rpsL31 xyl-5 mtl-1 arg3 thi-1 uvrA6 Δ(umuDC)595::cat fadR615::Tn10 purB5834
EC8thr-1 ara-14 leuB6 Δ(gpt-proA)62 lacY1 tsx-33 supE44 galK2 hisG4 rpsL31 xyl-5 mtl-1 argE3 thi-1 uvrA6 Δ(umuDC)596::ermGT fadR+ purB+11
KY1056sFtet101AB1157 derivative; harboring F′ derived from CC101, which has Tn10 in it for selection of F′K. Yamamoto
KY1056sFtet104AB1157 derivative; harboring F′ derived from CC104, which has Tn10 in it for selection of F′K. Yamamoto
YG6125AAB1157 derivative; harboring F′ derived from CC101, which has Tn10 in it for selection of F′ and ΔdinB::kanThis study
YG6125BAB1157 derivative; harboring F′ derived from CC104, which has Tn10 in it for the selection of F′ and ΔdinB::kanThis study
QC1736Δ(argF-lac)U169 rpsL ΔsodA3 sodB::MudPR fur::kan; Cmr Kmr29
YG6177Like QC1736 but ΔdinB61::ble; Cmr Kmr ZcrAR30 (P1) → QC1736This study
YG6180Like QC1736 but ΔumuDC(596)::ermGT; Cmr KmrDE2302/EC8 (P1) → QC1736This study
YG6124Like QC1736 ΔdinB61::ble; ΔumuDC(596)::ermGT; Cmr Kmr ZcrDE2302/EC8 (P1) → YG6177This study
YG6175bLike QC1736 but harboring F′ from CC101; Cmr Kmr TcrKY1056sFtet101 → QC1736This study
YG6176bLike QC1736 but harboring F′ from CC104; Cmr Kmr TcrKY1056sFtet104 → QC1736This study
YG6178bLike QC1736 but ΔdinB61::ble and harboring F′ from CC101; Cmr Kmr Tcr ZcrYG6125A → YG6177This study
YG6179bLike QC1736 but ΔdinB61::ble and harboring F′ from CC104; Cmr Kmr Tcr ZcrYG6125B → YG6177This study
YG6181bLike QC1736 but ΔumuDC(596)::ermGT and harboring F′ derived from CC101; Cmr Kmr TcrKY1056sFtet101 → YG6180This study
YG6182bLike QC1736 but ΔumuDC(596)::ermGT and harboring F′ from CC104; Cmr Kmr TcrKY1056sFtet104 → YG6180This study
YG6126bLike QC1736 but ΔdinB61::ble and ΔumuDC(596)::ermGT and harboring F′ from CC101; Cmr Kmr Tcr ZcrYG6125A → YG6124This study
YG6127bLike QC1736 but ΔdinB61::ble and ΔumuDC(596)::ermGT and harboring F′ from CC104; Cmr Kmr Tcr ZcrYG6125B → YG6124This study
  • a The deletion strains for dinB encoding DNA Pol IV were constructed by P1 transduction as indicated. The umuDC deletion encoding DNA Pol V was introduced into QC1736 and YG6177 by two-step P1 transduction (11). (P1) indicates that P1vir phage lysate was prepared in the strain. F′ with a mutation for specific detection of changes from G · C to T · A or from A · T to C · G was separately introduced by conjugation as indicated. The arrows indicate the directions of transfer for P1 transduction and conjugation. Chloramphenicol, kanamycin, tetracycline, and zeocin were used at concentrations of 10 μg/ml, 25 μg/ml, 10 μg/ml, and 50 μg/ml, respectively. Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance; Zcr, zeocin resistance.

  • b Strain used for the LacZ reversion assay.