TABLE 2.

Genes up-regulated in N. gonorrhoeae strain JKD5068 when RpoH is overexpressed

Open reading frameaGeneFold change Proposed functiond
MicroarraybRT-PCRc
NGO0288 rpoH 3.210.1RNA polymerase sigma factor
NGO1244e marR 2.38.9Transcriptional regulator MarR
NGO1046 clpB 2.35.1HSP, chaperone
NGO1429 dnaK 2.23.1HSP, chaperone
NGO1422 grpE 2.1HSP, chaperone
NGO0775 lon 2.0ATP-dependent protease
NGO1694 folA 2.0Dihydrofolate reductase
NGO0116 secB 2.02.7Protein secretion
NGO2094 groES 1.9HSP, chaperone
NGO12221.8OsmC-like family protein
NGO0570 creA 1.8DNA-binding protein
NGO1901 dnaJ 1.7HSP, chaperone
NGO14261.6Conserved hypothetical protein
  • a Designations from the annotation at www.ncbi.nlm.nih.gov/entrez/query.fcgi .

  • b Average expression ratio from microarray analysis for genes that were up-regulated at least 1.5-fold with a P value of ≤0.001.

  • c Expression ratio for genes that were analyzed by quantitative real-time RT-PCR.

  • d Proposed functions of the genes based on sequence annotation, sequence homology, and previous investigations. HSP, heat shock protein.

  • e P = 0.009.